biostudies-literatureUnknown171(21)Omori KMochida Memorial FoundationProgram for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN)JSPS FellowsTakeda Science FoundationScience and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food IndustryJapan Society for the Promotion of ScienceTowa Foundation<h4>Background and purpose</h4>PGE2 is a major prostanoid that regulates inflammation by stimulating EP1-4 receptors. However, how PGE2 induces an initial inflammatory response to vascular hyper-permeability remains unknown. Here we investigated the role of the PGE2 -EP receptor signal in modulating vascular permeability both in vivo and in vitro.<h4>Experimental approach</h4>We used a modified Miles assay and intravital microscopy to examine vascular permeability in vivo. Endothelial barrier property was assessed by measuring transendothelial electrical resistance (TER) in vitro.<h4>Key results</h4>Local administration of PGE2 , an EP2 or EP4 receptor agonist into FVB/NJcl mouse ear skin caused vascular leakage, indicated by dye extravasation. Intravital microscopy and laser Doppler bloodBritish journal of pharmacology4879-89https://www.ebi.ac.uk/biostudies/studies/S-EPMC4294111biostudies-literatureMultiple roles of the PGE2 -EP receptor signal in vascular permeability.PMC4294111Murata THori MOzaki HOmori KKida TfalseMultiple roles of the PGE2 -EP receptor signal in vascular permeability.<h4>Background and purpose</h4>PGE2 is a major prostanoid that regulates inflammation by stimulating EP1-4 receptors. However, how PGE2 induces an initial inflammatory response to vascular hyper-permeability remains unknown. Here we investigated the role of the PGE2 -EP receptor signal in modulating vascular permeability both in vivo and in vitro.<h4>Experimental approach</h4>We used a modified Miles assay and intravital microscopy to examine vascular permeability in vivo. Endothelial barrier property was assessed by measuring transendothelial electrical resistance (TER) in vitro.<h4>Key results</h4>Local administration of PGE2 , an EP2 or EP4 receptor agonist into FVB/NJcl mouse ear skin caused vascular leakage, indicated by dye extravasation. Intravital microscopy and laser Doppler blood2014-01-01T00:00:00Z2014 Nov2025-05-29T21:22:07.623Z2025-05-29T21:22:07.623ZS-EPMC42941112492377210.1111/bph.12815