{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Liu J"],"pubmed_abstract":["<h4>Background</h4>The virulent Newcastle disease virus (NDV) strain NA-1 (genotype VII) was isolated from an epizootic involving a flock of geese in Jilin Province, Northeast China, in 1999. Compared with the classical NDV strains, which have a genome size of 15,186 bp, the more recently isolated NDV strains, including that involved in the goose outbreak, have an extra six nucleotides in the 5'-untranslated region (UTR) of the nucleoprotein (NP) gene. This extra sequence, TCCCAC, is highly conserved and has been found in multiple NDV strains, including ZJ-1, WF00G, JSG0210, and NA-1. In the current study, an infectious clone from strain NA-1 was isolated and designated rNA-1. Subsequently, strain rNA-1 was mutated to delete the six-nucleotide insertion, producing strain rNA-1(-). Virulenc"],"journal":["BMC veterinary research"],"pagination":["964"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4297397"],"repository":["biostudies-literature"],"pubmed_title":["The deletion of an extra six nucleotides in the 5' -untranslated region of the nucleoprotein gene of Newcastle disease virus NA-1 decreases virulence."],"pmcid":["PMC4297397"],"pubmed_authors":["Liu X","Liu J","Ding C","Yu S","Wang C","Yin R","Cong Y","Ding Z"],"additional_accession":[]},"is_claimable":false,"name":"The deletion of an extra six nucleotides in the 5' -untranslated region of the nucleoprotein gene of Newcastle disease virus NA-1 decreases virulence.","description":"<h4>Background</h4>The virulent Newcastle disease virus (NDV) strain NA-1 (genotype VII) was isolated from an epizootic involving a flock of geese in Jilin Province, Northeast China, in 1999. Compared with the classical NDV strains, which have a genome size of 15,186 bp, the more recently isolated NDV strains, including that involved in the goose outbreak, have an extra six nucleotides in the 5'-untranslated region (UTR) of the nucleoprotein (NP) gene. This extra sequence, TCCCAC, is highly conserved and has been found in multiple NDV strains, including ZJ-1, WF00G, JSG0210, and NA-1. In the current study, an infectious clone from strain NA-1 was isolated and designated rNA-1. Subsequently, strain rNA-1 was mutated to delete the six-nucleotide insertion, producing strain rNA-1(-). Virulenc","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Dec","modification":"2025-04-22T15:18:26.637Z","creation":"2019-03-27T01:43:33Z"},"accession":"S-EPMC4297397","cross_references":{"pubmed":["25528581"],"doi":["10.1186/s12917-014-0305-5"]}}