{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Berry D"],"funding":["Österreichische Forschungsförderungsgesellschaft","U.S. Department of Energy","Austrian Science Fund FWF","European Research Council","Natural Environment Research Council","Engineering and Physical Sciences Research Council"],"pagination":["E194-203"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4299247"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["112(2)"],"pubmed_abstract":["Microbial communities are essential to the function of virtually all ecosystems and eukaryotes, including humans. However, it is still a major challenge to identify microbial cells active under natural conditions in complex systems. In this study, we developed a new method to identify and sort active microbes on the single-cell level in complex samples using stable isotope probing with heavy water (D2O) combined with Raman microspectroscopy. Incorporation of D2O-derived D into the biomass of autotrophic and heterotrophic bacteria and archaea could be unambiguously detected via C-D signature peaks in single-cell Raman spectra, and the obtained labeling pattern was confirmed by nanoscale-resolution secondary ion MS. In fast-growing Escherichia coli cells, label detection was already possible"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells."],"pmcid":["PMC4299247"],"funding_grant_id":["DE-AC02-05CH11231","EP/M002403/1","P26127-B20","P 26127","294343","NE/M002934/1","NITRICARE 294343"],"pubmed_authors":["Shterzer N","Decker T","Woebken D","Schmid MC","Lee TK","Wagner M","Berry D","Schintlmeister A","Zhu D","Mizrahi I","Rauch I","Huang WE","Fowler PW","Mader E","Palatinszky M","Wang Y","Gibson CM","Popp J","Hanson BT","Bocklitz T"],"additional_accession":[]},"is_claimable":false,"name":"Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells.","description":"Microbial communities are essential to the function of virtually all ecosystems and eukaryotes, including humans. However, it is still a major challenge to identify microbial cells active under natural conditions in complex systems. In this study, we developed a new method to identify and sort active microbes on the single-cell level in complex samples using stable isotope probing with heavy water (D2O) combined with Raman microspectroscopy. Incorporation of D2O-derived D into the biomass of autotrophic and heterotrophic bacteria and archaea could be unambiguously detected via C-D signature peaks in single-cell Raman spectra, and the obtained labeling pattern was confirmed by nanoscale-resolution secondary ion MS. In fast-growing Escherichia coli cells, label detection was already possible","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jan","modification":"2026-04-30T23:13:23.832Z","creation":"2019-03-27T01:43:41Z"},"accession":"S-EPMC4299247","cross_references":{"pubmed":["25550518"],"doi":["10.1073/pnas.1420406112"]}}