{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(9)"],"submitter":["Mirjamali NA"],"pubmed_abstract":["<h4>Objectives</h4>Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity.<h4>Materials and methods</h4>The enzymatic region of hyaluronidase gene was detected by bioinformatics method. The PCR method was used to amplify enzymatic region of hyaluronidase gene from chromosomal DNA of Streptococcus pyogenes. The eluted product was cloned into the prokaryotic expression vector pET32a which was digested by BamHI and HindIII restriction endonuclease enzymes. The target protein was expressed in the Escherichia coli. The bacteria including pET32a-hylA"],"journal":["Iranian journal of basic medical sciences"],"pagination":["667-72"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4322150"],"repository":["biostudies-literature"],"pubmed_title":["Cloning and expression of the enzymatic region of Streptococcal hyaluronidase."],"pmcid":["PMC4322150"],"pubmed_authors":["Mirjamali NA","Soufian S","Molaee N","Abbasian SS","Abtahi H"],"additional_accession":[]},"is_claimable":false,"name":"Cloning and expression of the enzymatic region of Streptococcal hyaluronidase.","description":"<h4>Objectives</h4>Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity.<h4>Materials and methods</h4>The enzymatic region of hyaluronidase gene was detected by bioinformatics method. The PCR method was used to amplify enzymatic region of hyaluronidase gene from chromosomal DNA of Streptococcus pyogenes. The eluted product was cloned into the prokaryotic expression vector pET32a which was digested by BamHI and HindIII restriction endonuclease enzymes. The target protein was expressed in the Escherichia coli. The bacteria including pET32a-hylA","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Sep","modification":"2025-04-19T02:04:53.157Z","creation":"2019-03-27T01:44:54Z"},"accession":"S-EPMC4322150","cross_references":{"pubmed":["25691943"]}}