biostudies-literatureUnknown56(2)Sakamoto SThe secondary cell wall constitutes a rigid frame of cells in plant tissues where rigidity is required. Deposition of the secondary cell wall in fiber cells contributes to the production of wood in woody plants. The secondary cell wall is assembled through co-operative activities of many enzymes, and their gene expression is precisely regulated by a pyramidal cascade of transcription factors. Deposition of a transmuted secondary cell wall in empty fiber cells by expressing selected gene(s) in this cascade has not been attempted previously. In this proof-of-concept study, we expressed chimeric activators of 24 transcription factors that are preferentially expressed in the stem, in empty fiber cells of the Arabidopsis nst1-1 nst3-1 double mutant, which lacks a secondary cell wall in fiber cePlant & cell physiology299-310https://www.ebi.ac.uk/biostudies/studies/S-EPMC4323883biostudies-literatureReconstitution of a secondary cell wall in a secondary cell wall-deficient Arabidopsis mutant.PMC4323883Mitsuda NSakamoto SfalseReconstitution of a secondary cell wall in a secondary cell wall-deficient Arabidopsis mutant.The secondary cell wall constitutes a rigid frame of cells in plant tissues where rigidity is required. Deposition of the secondary cell wall in fiber cells contributes to the production of wood in woody plants. The secondary cell wall is assembled through co-operative activities of many enzymes, and their gene expression is precisely regulated by a pyramidal cascade of transcription factors. Deposition of a transmuted secondary cell wall in empty fiber cells by expressing selected gene(s) in this cascade has not been attempted previously. In this proof-of-concept study, we expressed chimeric activators of 24 transcription factors that are preferentially expressed in the stem, in empty fiber cells of the Arabidopsis nst1-1 nst3-1 double mutant, which lacks a secondary cell wall in fiber ce2015-01-01T00:00:00Z2015 Feb2025-04-04T22:04:13.873Z2019-03-27T01:45:00ZS-EPMC43238832553519510.1093/pcp/pcu208