biostudies-literatureUnknown16Zhang Y<h4>Background</h4>Intestinal microbes play significant roles in fish and can be possibly used as probiotics in aquaculture. In our previous study, Flaviramulus ichthyoenteri Th78(T), a novel species in the family Flavobacteriaceae, was isolated from fish intestine and showed strong quorum quenching (QQ) ability. To identify the QQ enzymes in Th78(T) and explore the potential roles of Th78(T) in fish intestine, we sequenced the genome of Th78(T) and performed extensive genomic analysis.<h4>Results</h4>An N-acyl homoserine lactonase FiaL belonging to the metallo-β-lactamase superfamily was identified and the QQ activity of heterologously expressed FiaL was confirmed in vitro. FiaL has relatively little similarity to the known lactonases (25.2 ~ 27.9% identity in amino acid sequence). VariouBMC genomics38https://www.ebi.ac.uk/biostudies/studies/S-EPMC4324048biostudies-literatureGenome analysis of Flaviramulus ichthyoenteri Th78(T) in the family Flavobacteriaceae: insights into its quorum quenching property and potential roles in fish intestine.PMC4324048Tang KZhang XHLiu JYu MZhang YCoenye TfalseGenome analysis of Flaviramulus ichthyoenteri Th78(T) in the family Flavobacteriaceae: insights into its quorum quenching property and potential roles in fish intestine.<h4>Background</h4>Intestinal microbes play significant roles in fish and can be possibly used as probiotics in aquaculture. In our previous study, Flaviramulus ichthyoenteri Th78(T), a novel species in the family Flavobacteriaceae, was isolated from fish intestine and showed strong quorum quenching (QQ) ability. To identify the QQ enzymes in Th78(T) and explore the potential roles of Th78(T) in fish intestine, we sequenced the genome of Th78(T) and performed extensive genomic analysis.<h4>Results</h4>An N-acyl homoserine lactonase FiaL belonging to the metallo-β-lactamase superfamily was identified and the QQ activity of heterologously expressed FiaL was confirmed in vitro. FiaL has relatively little similarity to the known lactonases (25.2 ~ 27.9% identity in amino acid sequence). Variou2015-01-01T00:00:00Z2015 Feb2026-06-09T05:56:25.994Z2019-03-27T01:45:01ZS-EPMC43240482565284610.1186/s12864-015-1275-0