biostudies-literatureZirin JNIDDK NIH HHSHoward Hughes Medical InstituteNCI NIH HHSNIAMS NIH HHSNIGMS NIH HHSe1005006https://www.ebi.ac.uk/biostudies/studies/S-EPMC4334200biostudies-literatureUnknown11(2)Given the diversity of autophagy targets and regulation, it is important to characterize autophagy in various cell types and conditions. We used a primary myocyte cell culture system to assay the role of putative autophagy regulators in the specific context of skeletal muscle. By treating the cultures with rapamycin (Rap) and chloroquine (CQ) we induced an autophagic response, fully suppressible by knockdown of core ATG genes. We screened D. melanogaster orthologs of a previously reported mammalian autophagy protein-protein interaction network, identifying several proteins required for autophagosome formation in muscle cells, including orthologs of the Rab regulators RabGap1 and Rab3Gap1. The screen also highlighted the critical roles of the proteasome and glycogen metabolism in regulatingPLoS geneticsRegulators of autophagosome formation in Drosophila muscles.PMC4334200F32GM082174-02R01 AR057352R01 DK088718F32 GM082174AR057352P01CA120964P01 CA120964Perrimon NZirin JNieuwenhuis JSamsonova ATao RfalseRegulators of autophagosome formation in Drosophila muscles.Given the diversity of autophagy targets and regulation, it is important to characterize autophagy in various cell types and conditions. We used a primary myocyte cell culture system to assay the role of putative autophagy regulators in the specific context of skeletal muscle. By treating the cultures with rapamycin (Rap) and chloroquine (CQ) we induced an autophagic response, fully suppressible by knockdown of core ATG genes. We screened D. melanogaster orthologs of a previously reported mammalian autophagy protein-protein interaction network, identifying several proteins required for autophagosome formation in muscle cells, including orthologs of the Rab regulators RabGap1 and Rab3Gap1. The screen also highlighted the critical roles of the proteasome and glycogen metabolism in regulating2015-01-01T00:00:00Z2015 Feb2025-05-29T21:21:45.408Z2025-05-29T21:21:45.408ZS-EPMC43342002569268410.1371/journal.pgen.1005006