biostudies-literatureUnknown12(2)Skora JThe aim of the study was to evaluate the ability of Alternaria isolates from workplaces to produce Alt a 1 allergenic protein, and to analyze whether technical materials (cellulose, compost, leather) present within the working environment stimulate or inhibit Alt a 1 production (ELISA test). Studies included identification of the isolated molds by nucleotide sequences analyzing of the ITS1/ITS2 regions, actin, calmodulin and Alt a 1 genes. It has been shown that Alternaria molds are significant part of microbiocenosis in the archive, museum, library, composting plant and tannery (14%-16% frequency in the air). The presence of the gene encoding the Alt a 1 protein has been detected for the strains: Alternaria alternata, A. lini, A. limoniasperae A. nobilis and A. tenuissima. Environmental sInternational journal of environmental research and public health2164-83https://www.ebi.ac.uk/biostudies/studies/S-EPMC4344718biostudies-literatureProduction of the allergenic protein Alt a 1 by Alternaria isolates from working environments.PMC4344718Majak IStepien LOtlewska ALeszczynska JGutarowska BSkora JfalseProduction of the allergenic protein Alt a 1 by Alternaria isolates from working environments.The aim of the study was to evaluate the ability of Alternaria isolates from workplaces to produce Alt a 1 allergenic protein, and to analyze whether technical materials (cellulose, compost, leather) present within the working environment stimulate or inhibit Alt a 1 production (ELISA test). Studies included identification of the isolated molds by nucleotide sequences analyzing of the ITS1/ITS2 regions, actin, calmodulin and Alt a 1 genes. It has been shown that Alternaria molds are significant part of microbiocenosis in the archive, museum, library, composting plant and tannery (14%-16% frequency in the air). The presence of the gene encoding the Alt a 1 protein has been detected for the strains: Alternaria alternata, A. lini, A. limoniasperae A. nobilis and A. tenuissima. Environmental s2015-01-01T00:00:00Z2015 Feb2025-04-19T16:45:53.656Z2020-11-19T13:25:07ZS-EPMC43447182568999410.3390/ijerph120202164