biostudies-literatureDejda ACanadian Institutes of Health Research4807-22https://www.ebi.ac.uk/biostudies/studies/S-EPMC4347252biostudies-literatureUnknown124(11)Immunological activity in the CNS is largely dependent on an innate immune response and is heightened in diseases, such as diabetic retinopathy, multiple sclerosis, amyotrophic lateral sclerosis, and Alzheimer's disease. The molecular dynamics governing immune cell recruitment to sites of injury and disease in the CNS during sterile inflammation remain poorly defined. Here, we identified a subset of mononuclear phagocytes (MPs) that responds to local chemotactic cues that are conserved among central neurons, vessels, and immune cells. Patients suffering from late-stage proliferative diabetic retinopathy (PDR) had elevated vitreous semaphorin 3A (SEMA3A). Using a murine model, we found that SEMA3A acts as a potent attractant for neuropilin-1-positive (NRP-1-positive) MPs. These proangiogeniThe Journal of clinical investigationNeuropilin-1 mediates myeloid cell chemoattraction and influences retinal neuroimmune crosstalk.PMC4347252221478Beaudoin FDejda AMawambo GRezende FALabrecque NAkla NDaudelin JFBoulet SSapieha PMenard COubaha MShao ZHenriques SDelisle JSCerani AStahl AMiloudi KfalseNeuropilin-1 mediates myeloid cell chemoattraction and influences retinal neuroimmune crosstalk.Immunological activity in the CNS is largely dependent on an innate immune response and is heightened in diseases, such as diabetic retinopathy, multiple sclerosis, amyotrophic lateral sclerosis, and Alzheimer's disease. The molecular dynamics governing immune cell recruitment to sites of injury and disease in the CNS during sterile inflammation remain poorly defined. Here, we identified a subset of mononuclear phagocytes (MPs) that responds to local chemotactic cues that are conserved among central neurons, vessels, and immune cells. Patients suffering from late-stage proliferative diabetic retinopathy (PDR) had elevated vitreous semaphorin 3A (SEMA3A). Using a murine model, we found that SEMA3A acts as a potent attractant for neuropilin-1-positive (NRP-1-positive) MPs. These proangiogeni2014-01-01T00:00:00Z2014 Nov2025-04-04T09:28:08.61Z2019-03-27T01:47:27ZS-EPMC43472522527162510.1172/JCI7649210.1172/jci76492