{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Muthana MM"],"funding":["NIGMS NIH HHS"],"pagination":["4595-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4348237"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["51(22)"],"pubmed_abstract":["Arabidopsis thaliana glucuronokinase (AtGlcAK) was cloned and shown to be able to use various uronic acids as substrates to produce the corresponding uronic acid-1-phosphates. AtGlcAK or Bifidobacterium infantis galactokinase (BiGalK) was used with a UDP-sugar pyrophosphorylase, an inorganic pyrophosphatase, with or without a glycosyltransferase for highly efficient synthesis of UDP-uronic acids and glucuronides. These improved cost-effective one-pot multienzyme (OPME) systems avoid the use of nicotinamide adenine dinucleotide (NAD(+))-cofactor in dehydrogenase-dependent UDP-glucuronic acid production processes and can be broadly applied for synthesizing various glucuronic acid-containing molecules."],"journal":["Chemical communications (Cambridge, England)"],"pubmed_title":["Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides."],"pmcid":["PMC4348237"],"funding_grant_id":["R01 GM094523","R01GM094523"],"pubmed_authors":["Li Y","Siu A","Wang PG","Muthana MM","Qu J","Xue M","Zhang L","Chen X","Klyuchnik T","Li L","Yu H"],"additional_accession":[]},"is_claimable":false,"name":"Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides.","description":"Arabidopsis thaliana glucuronokinase (AtGlcAK) was cloned and shown to be able to use various uronic acids as substrates to produce the corresponding uronic acid-1-phosphates. AtGlcAK or Bifidobacterium infantis galactokinase (BiGalK) was used with a UDP-sugar pyrophosphorylase, an inorganic pyrophosphatase, with or without a glycosyltransferase for highly efficient synthesis of UDP-uronic acids and glucuronides. These improved cost-effective one-pot multienzyme (OPME) systems avoid the use of nicotinamide adenine dinucleotide (NAD(+))-cofactor in dehydrogenase-dependent UDP-glucuronic acid production processes and can be broadly applied for synthesizing various glucuronic acid-containing molecules.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Mar","modification":"2020-10-29T13:02:46Z","creation":"2019-03-27T01:47:29Z"},"accession":"S-EPMC4348237","cross_references":{"pubmed":["25686901"],"doi":["10.1039/c4cc10306h"]}}