{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang X"],"funding":["NIGMS NIH HHS"],"pagination":["724-38"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4349990"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(3)"],"pubmed_abstract":["The challenge in high-quality membrane proteomics is all about sample preparation prior to HPLC, and the cell-to-protein step poses a long-standing bottleneck. Traditional protein extraction methods apply ionic or poly-disperse detergents, harsh denaturation, and repeated protein/peptide precipitation/resolubilization afterward, but suffer low yield, low reproducibility, and low sequence coverage. Contrary to attempts to subdue, we resolved this challenge by providing proteins nature-and-activity-promoting conditions throughout preparation. Using 285-kDa hetero-pentameric human GABA type A receptor overexpressed in HEK293 as a model, we describe a n-dodecyl-β-d-maltopyranoside/cholesteryl hemisuccinate (DDM/CHS)-based affinity purification method, that produced active receptors, supported "],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["Dodecyl maltopyranoside enabled purification of active human GABA type A receptors for deep and direct proteomic sequencing."],"pmcid":["PMC4349990"],"funding_grant_id":["P01 GM058448","GM 58448,"],"pubmed_authors":["Miller KW","Zhang X"],"additional_accession":[]},"is_claimable":false,"name":"Dodecyl maltopyranoside enabled purification of active human GABA type A receptors for deep and direct proteomic sequencing.","description":"The challenge in high-quality membrane proteomics is all about sample preparation prior to HPLC, and the cell-to-protein step poses a long-standing bottleneck. Traditional protein extraction methods apply ionic or poly-disperse detergents, harsh denaturation, and repeated protein/peptide precipitation/resolubilization afterward, but suffer low yield, low reproducibility, and low sequence coverage. Contrary to attempts to subdue, we resolved this challenge by providing proteins nature-and-activity-promoting conditions throughout preparation. Using 285-kDa hetero-pentameric human GABA type A receptor overexpressed in HEK293 as a model, we describe a n-dodecyl-β-d-maltopyranoside/cholesteryl hemisuccinate (DDM/CHS)-based affinity purification method, that produced active receptors, supported ","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Mar","modification":"2026-04-16T21:17:56.72Z","creation":"2019-03-27T01:47:34Z"},"accession":"S-EPMC4349990","cross_references":{"pubmed":["25473089"],"doi":["10.1074/mcp.M114.042556","10.1074/mcp.m114.042556"]}}