{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pangrsic T"],"funding":["Bundesministerium für Bildung und Forschung","Deutsche Forschungsgemeinschaft","Alexander von Humboldt-Stiftung"],"pagination":["E1028-37"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4352837"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["112(9)"],"pubmed_abstract":["EF-hand Ca(2+)-binding proteins are thought to shape the spatiotemporal properties of cellular Ca(2+) signaling and are prominently expressed in sensory hair cells in the ear. Here, we combined genetic disruption of parvalbumin-α, calbindin-D28k, and calretinin in mice with patch-clamp recording, in vivo physiology, and mathematical modeling to study their role in Ca(2+) signaling, exocytosis, and sound encoding at the synapses of inner hair cells (IHCs). IHCs lacking all three proteins showed excessive exocytosis during prolonged depolarizations, despite enhanced Ca(2+)-dependent inactivation of their Ca(2+) current. Exocytosis of readily releasable vesicles remained unchanged, in accordance with the estimated tight spatial coupling of Ca(2+) channels and release sites (effective \"couplin"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells."],"pmcid":["PMC4352837"],"funding_grant_id":["CRC889","01GQ1005A","fellowship","01GQ0811"],"pubmed_authors":["Gabrielaitis M","Strenzke N","Michanski S","Pangrsic T","Wolf F","Schwaller B","Moser T"],"additional_accession":[]},"is_claimable":false,"name":"EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.","description":"EF-hand Ca(2+)-binding proteins are thought to shape the spatiotemporal properties of cellular Ca(2+) signaling and are prominently expressed in sensory hair cells in the ear. Here, we combined genetic disruption of parvalbumin-α, calbindin-D28k, and calretinin in mice with patch-clamp recording, in vivo physiology, and mathematical modeling to study their role in Ca(2+) signaling, exocytosis, and sound encoding at the synapses of inner hair cells (IHCs). IHCs lacking all three proteins showed excessive exocytosis during prolonged depolarizations, despite enhanced Ca(2+)-dependent inactivation of their Ca(2+) current. Exocytosis of readily releasable vesicles remained unchanged, in accordance with the estimated tight spatial coupling of Ca(2+) channels and release sites (effective \"couplin","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Mar","modification":"2025-04-18T15:30:14.286Z","creation":"2019-03-27T01:47:47Z"},"accession":"S-EPMC4352837","cross_references":{"pubmed":["25691754"],"doi":["10.1073/pnas.1416424112"]}}