{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(42)"],"submitter":["Hong Y"],"pubmed_abstract":["AIM:To construct the plasmid pcHEV23 containing fragments of HEV ORF2 and ORF3 chimeric gene and to assess its ability to elicit specific immunologic response in mice. METHODS:The gene encoding the structural protein of HEV ORF2 fragment and full-length ORF3 was amplified by PCR. The PCR products were cloned into an eucaryotic expression plasmid pcDNA3. The resulting plasmid pcHEV23 was used as a DNA vaccine to inoculate BALB/c mice intramuscularly thrice at a dose of 100 or 200 microg. Mice injected with empty pcDNA3 DNA or saline served as control and then specific immune responses in the mice were detected. RESULTS:After 2-3 times of inoculation, all mice injected with pcHEV23 had anti-HEV IgG seroconversion and specific T lymphocyte proliferation. The lymphocyte stimulation index in the group immunized with pcHEV23 (3.1+/-0.49) was higher than that in the control group (0.787+/-0.12, P<0.01). None in the control group had a detectable level of anti-HEV IgG. CONCLUSION:DNA vaccine containing HEV ORF2 and ORF3 chimeric gene can successfully induce specific humoral and cellular immune response in mice."],"journal":["World journal of gastroenterology"],"pagination":["6713-5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4355771"],"repository":["biostudies-literature"],"pubmed_title":["Hepatitis E virus chimeric DNA vaccine elicits immunologic response in mice."],"pmcid":["PMC4355771"],"pubmed_authors":["Hong Y","Yang LH","Jing L","Ruan B","Wang YT","Hu HJ","Chen Y"],"additional_accession":[]},"is_claimable":false,"name":"Hepatitis E virus chimeric DNA vaccine elicits immunologic response in mice.","description":"AIM:To construct the plasmid pcHEV23 containing fragments of HEV ORF2 and ORF3 chimeric gene and to assess its ability to elicit specific immunologic response in mice. METHODS:The gene encoding the structural protein of HEV ORF2 fragment and full-length ORF3 was amplified by PCR. The PCR products were cloned into an eucaryotic expression plasmid pcDNA3. The resulting plasmid pcHEV23 was used as a DNA vaccine to inoculate BALB/c mice intramuscularly thrice at a dose of 100 or 200 microg. Mice injected with empty pcDNA3 DNA or saline served as control and then specific immune responses in the mice were detected. RESULTS:After 2-3 times of inoculation, all mice injected with pcHEV23 had anti-HEV IgG seroconversion and specific T lymphocyte proliferation. The lymphocyte stimulation index in the group immunized with pcHEV23 (3.1+/-0.49) was higher than that in the control group (0.787+/-0.12, P<0.01). None in the control group had a detectable level of anti-HEV IgG. CONCLUSION:DNA vaccine containing HEV ORF2 and ORF3 chimeric gene can successfully induce specific humoral and cellular immune response in mice.","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Nov","modification":"2020-11-19T15:31:45Z","creation":"2019-03-27T01:47:58Z"},"accession":"S-EPMC4355771","cross_references":{"pubmed":["16425371"],"doi":["10.3748/wjg.v11.i42.6713"]}}