{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3(2)"],"submitter":["Cheng YB"],"pubmed_abstract":["<i>N</i>-methyl-<i>N</i>'-nitro-<i>N</i>-nitrosoguanidine (MNNG) is an alkylating agent that can induce gastric carcinoma. As a well-known human carcinogen, MNNG has been universally recognized as a methylating agent and is believed to act through methylation mechanism. In the present study, the epigenetic status of the human telomerase reverse transcriptase (hTERT) promoter was investigated in MNNG-treated normal human gastric mucosal epithelial cells. After 4 h exposure to MNNG at different concentrations, 6.8 and 68 µM, bisulfite sequencing polymerase chain reaction showed that five methylated cytosines outside the CpG dinucleotides in the 290-bp fragment from the hTERT promoter were demethylated and all the methylated cytosines in CpG dinucleotides remained intact. Furthermore, the epi"],"journal":["Biomedical reports"],"pagination":["176-178"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4360657"],"repository":["biostudies-literature"],"pubmed_title":["Demethylation of the hTERT promoter in normal human gastric mucosal epithelial cells following <i>N</i>-methyl-<i>N</i>'-nitro-<i>N</i>-nitrosoguanidine exposure."],"pmcid":["PMC4360657"],"pubmed_authors":["Yao P","Guo LP","Fang DC","Cheng YB","Ning XY","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Demethylation of the hTERT promoter in normal human gastric mucosal epithelial cells following <i>N</i>-methyl-<i>N</i>'-nitro-<i>N</i>-nitrosoguanidine exposure.","description":"<i>N</i>-methyl-<i>N</i>'-nitro-<i>N</i>-nitrosoguanidine (MNNG) is an alkylating agent that can induce gastric carcinoma. As a well-known human carcinogen, MNNG has been universally recognized as a methylating agent and is believed to act through methylation mechanism. In the present study, the epigenetic status of the human telomerase reverse transcriptase (hTERT) promoter was investigated in MNNG-treated normal human gastric mucosal epithelial cells. After 4 h exposure to MNNG at different concentrations, 6.8 and 68 µM, bisulfite sequencing polymerase chain reaction showed that five methylated cytosines outside the CpG dinucleotides in the 290-bp fragment from the hTERT promoter were demethylated and all the methylated cytosines in CpG dinucleotides remained intact. Furthermore, the epi","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Mar","modification":"2025-04-22T19:54:45.367Z","creation":"2019-06-06T14:07:49Z"},"accession":"S-EPMC4360657","cross_references":{"pubmed":["25798244"],"doi":["10.3892/br.2014.398"]}}