{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Madisen L"],"funding":["NIDA NIH HHS","NIMH NIH HHS","Wellcome Trust"],"pagination":["942-58"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4365051"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["85(5)"],"pubmed_abstract":["An increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at subst"],"journal":["Neuron"],"pubmed_title":["Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance."],"pmcid":["PMC4365051"],"funding_grant_id":["DA028298","MH085500","R01 MH085500","R01 DA028298","095669"],"pubmed_authors":["Sunkin SM","Garner AR","Nagy A","Carandini M","Klapoetke NC","Egolf L","Knopfel T","Shimaoka D","Gu H","Helmchen F","Tasic B","Nguyen TN","Miyawaki A","Monetti C","Niino Y","Cheng A","Empson RM","Madisen L","Li L","Reid RC","Benucci A","Boyden ES","van der Bourg A","Mills M","Chuong AS","Zeng H"],"additional_accession":[]},"is_claimable":false,"name":"Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.","description":"An increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA-dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at subst","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Mar","modification":"2025-04-19T14:59:11.074Z","creation":"2019-03-27T01:48:33Z"},"accession":"S-EPMC4365051","cross_references":{"pubmed":["25741722"],"doi":["10.1016/j.neuron.2015.02.022"]}}