{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5"],"submitter":["Xie X"],"pubmed_abstract":["MicroRNA (miRNA) studies are experiencing a transition from basic research applications to clinical applications. However, the lack of reliable and sensitive miRNA detection methods has become a bottleneck in the process. Here, we report an absolute quantification method based on the competitive PCR amplification of specific miRNAs and synthetic RNA spike-ins in a single reaction. RNA spike-ins are quantified as dynamic RNA copy number standards and are used to measure selected miRNAs free from the effects of intra-assay variables, including those from individual sample sources. Combined with the size differentiation power of capillary electrophoresis, the content of miRNAs was reproducibly measured, with verifiable detection limits of 10-46 copies over 5-log detection ranges. The direct measurements of miRNAs from 168 human serum samples and their considerable value as a diagnostic for bronchopneumonia and bronchiolitis demonstrate the potential of the assay in clinical applications."],"journal":["Scientific reports"],"pagination":["9356"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4366852"],"repository":["biostudies-literature"],"pubmed_title":["MicroRNA-derived fragment length polymorphism assay."],"pmcid":["PMC4366852"],"pubmed_authors":["Zhang F","Xu K","Yang Z","Yang Y","Feng Z","Zhu J","Xie X","Zhang Y","Tang F","Wu X"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-derived fragment length polymorphism assay.","description":"MicroRNA (miRNA) studies are experiencing a transition from basic research applications to clinical applications. However, the lack of reliable and sensitive miRNA detection methods has become a bottleneck in the process. Here, we report an absolute quantification method based on the competitive PCR amplification of specific miRNAs and synthetic RNA spike-ins in a single reaction. RNA spike-ins are quantified as dynamic RNA copy number standards and are used to measure selected miRNAs free from the effects of intra-assay variables, including those from individual sample sources. Combined with the size differentiation power of capillary electrophoresis, the content of miRNAs was reproducibly measured, with verifiable detection limits of 10-46 copies over 5-log detection ranges. The direct measurements of miRNAs from 168 human serum samples and their considerable value as a diagnostic for bronchopneumonia and bronchiolitis demonstrate the potential of the assay in clinical applications.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Mar","modification":"2026-05-03T06:51:18.581Z","creation":"2026-04-07T18:57:40.543Z"},"accession":"S-EPMC4366852","cross_references":{"pubmed":["25790971"],"doi":["10.1038/srep09356"]}}