{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fung SY"],"funding":["NHGRI NIH HHS","NIGMS NIH HHS","CIHR"],"pagination":["247-57"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4371607"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(1)"],"pubmed_abstract":["Toll-like receptors (TLRs) play a critical role in innate immunity, but activation of TLR signaling pathways is also associated with many harmful inflammatory diseases. Identification of novel anti-inflammatory molecules targeting TLR signaling pathways is central to the development of new treatment approaches for acute and chronic inflammation. We performed high-throughput screening from crude marine sponge extracts on TLR5 signaling and identified girolline. We demonstrated that girolline inhibits signaling through both MyD88-dependent and -independent TLRs (i.e., TLR2, 3, 4, 5, and 7) and reduces cytokine (IL-6 and IL-8) production in human peripheral blood mononuclear cells and macrophages. Using a chemical genomics approach, we identified Elongation Factor 2 as the molecular target of"],"journal":["ACS chemical biology"],"pubmed_title":["Unbiased screening of marine sponge extracts for anti-inflammatory agents combined with chemical genomics identifies girolline as an inhibitor of protein synthesis."],"pmcid":["PMC4371607"],"funding_grant_id":["1R01HG005084-01A1","MOP-57830","R01 GM104975","R01 HG005084","1R01GM104975-01"],"pubmed_authors":["Woods K","Fung SY","Piotrowski JS","Victor RE","Boone C","Li SC","Myers CL","de Voogd NJ","Sofiyev V","Deshpande R","Hirschfeld AF","Andersen RJ","Schneiderman J","Turvey SE"],"additional_accession":[]},"is_claimable":false,"name":"Unbiased screening of marine sponge extracts for anti-inflammatory agents combined with chemical genomics identifies girolline as an inhibitor of protein synthesis.","description":"Toll-like receptors (TLRs) play a critical role in innate immunity, but activation of TLR signaling pathways is also associated with many harmful inflammatory diseases. Identification of novel anti-inflammatory molecules targeting TLR signaling pathways is central to the development of new treatment approaches for acute and chronic inflammation. We performed high-throughput screening from crude marine sponge extracts on TLR5 signaling and identified girolline. We demonstrated that girolline inhibits signaling through both MyD88-dependent and -independent TLRs (i.e., TLR2, 3, 4, 5, and 7) and reduces cytokine (IL-6 and IL-8) production in human peripheral blood mononuclear cells and macrophages. Using a chemical genomics approach, we identified Elongation Factor 2 as the molecular target of","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014 Jan","modification":"2025-04-18T19:18:19.691Z","creation":"2019-03-27T01:48:41Z"},"accession":"S-EPMC4371607","cross_references":{"pubmed":["24117378"],"doi":["10.1021/cb400740c"]}}