biostudies-literatureIqbal KNIEHS NIH HHSNCI NIH HHSNIGMS NIH HHS59https://www.ebi.ac.uk/biostudies/studies/S-EPMC4376074biostudies-literatureUnknown16<h4>Background</h4>Exposure to environmental endocrine-disrupting chemicals during pregnancy reportedly causes transgenerationally inherited reproductive defects. We hypothesized that to affect the grandchild, endocrine-disrupting chemicals must alter the epigenome of the germ cells of the in utero-exposed G1 male fetus. Additionally, to affect the great-grandchild, the aberration must persist in the germ cells of the unexposed G2 grandchild.<h4>Results</h4>Here, we treat gestating female mice with vinclozolin, bisphenol A, or di-(2-ethylhexyl)phthalate during the time when global de novo DNA methylation and imprint establishment occurs in the germ cells of the G1 male fetus. We map genome-wide features in purified G1 and G2 prospermatogonia, in order to detect immediate and persistent epiGenome biologyDeleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming.PMC4376074R01ES015185R01GM064378P30 CA033572R01 ES015185R01 GM064378Warden CLi AXBai AYSingh PSzabo PEIqbal KPfeifer GPTran DAWu XfalseDeleterious effects of endocrine disruptors are corrected in the mammalian germline by epigenome reprogramming.<h4>Background</h4>Exposure to environmental endocrine-disrupting chemicals during pregnancy reportedly causes transgenerationally inherited reproductive defects. We hypothesized that to affect the grandchild, endocrine-disrupting chemicals must alter the epigenome of the germ cells of the in utero-exposed G1 male fetus. Additionally, to affect the great-grandchild, the aberration must persist in the germ cells of the unexposed G2 grandchild.<h4>Results</h4>Here, we treat gestating female mice with vinclozolin, bisphenol A, or di-(2-ethylhexyl)phthalate during the time when global de novo DNA methylation and imprint establishment occurs in the germ cells of the G1 male fetus. We map genome-wide features in purified G1 and G2 prospermatogonia, in order to detect immediate and persistent epi2015-01-01T00:00:00Z2015 Mar2026-06-14T05:26:11.857Z2026-06-14T03:09:26.882ZS-EPMC43760742585343310.1186/s13059-015-0619-z