biostudies-literatureMessner PAustrian Science Fund FWF1934-51https://www.ebi.ac.uk/biostudies/studies/S-EPMC4381302biostudies-literatureUnknown343(12)Cell surface layers (S-layers) are common structures of the bacterial cell envelope with a lattice-like appearance that are formed by a self-assembly process. Frequently, the constituting S-layer proteins are modified with covalently linked glycan chains facing the extracellular environment. S-layer glycoproteins from organisms of the Bacillaceae family possess long, O-glycosidically linked glycans that are composed of a great variety of sugar constituents. The observed variations already exceed the display found in eukaryotic glycoproteins. Recent investigations of the S-layer protein glycosylation process at the molecular level, which has lagged behind the structural studies due to the lack of suitable molecular tools, indicated that the S-layer glycoprotein glycan biosynthesis pathway uCarbohydrate researchS-layer nanoglycobiology of bacteria.PMC4381302P 18013P 19047Messner PSchaffer CSteiner KZarschler KfalseS-layer nanoglycobiology of bacteria.Cell surface layers (S-layers) are common structures of the bacterial cell envelope with a lattice-like appearance that are formed by a self-assembly process. Frequently, the constituting S-layer proteins are modified with covalently linked glycan chains facing the extracellular environment. S-layer glycoproteins from organisms of the Bacillaceae family possess long, O-glycosidically linked glycans that are composed of a great variety of sugar constituents. The observed variations already exceed the display found in eukaryotic glycoproteins. Recent investigations of the S-layer protein glycosylation process at the molecular level, which has lagged behind the structural studies due to the lack of suitable molecular tools, indicated that the S-layer glycoprotein glycan biosynthesis pathway u2008-01-01T00:00:00Z2008 Aug2025-04-06T23:58:18.642Z2019-03-27T01:49:10ZS-EPMC43813021833680110.1016/j.carres.2007.12.025