{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4"],"submitter":["Alon S"],"funding":["United States–Israel Binational Science Foundation","John and Donna Krenicki Endowment Fund","European Research Council","Israel Science Foundation","Marine Biological Laboratory","Clore Israel Foundation","National Institutes of Health","National Science Foundation"],"pubmed_abstract":["RNA editing by adenosine deamination alters genetic information from the genomic blueprint. When it recodes mRNAs, it gives organisms the option to express diverse, functionally distinct, protein isoforms. All eumetazoans, from cnidarians to humans, express RNA editing enzymes. However, transcriptome-wide screens have only uncovered about 25 transcripts harboring conserved recoding RNA editing sites in mammals and several hundred recoding sites in Drosophila. These studies on few established models have led to the general assumption that recoding by RNA editing is extremely rare. Here we employ a novel bioinformatic approach with extensive validation to show that the squid Doryteuthis pealeii recodes proteins by RNA editing to an unprecedented extent. We identify 57,108 recoding sites in the nervous system, affecting the majority of the proteins studied. Recoding is tissue-dependent, and enriched in genes with neuronal and cytoskeletal functions, suggesting it plays an important role in brain physiology."],"journal":["eLife"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4384741"],"repository":["biostudies-literature"],"pubmed_title":["The majority of transcripts in the squid nervous system are extensively recoded by A-to-I RNA editing."],"pmcid":["PMC4384741"],"funding_grant_id":["2013094","grant no. 311257","HRD-1137725","311257","R01 NS064259NIH","[379/12]","Graduate Student Fellowship"],"pubmed_authors":["Rosenthal JJ","Eisenberg E","Levanon EY","Graveley BR","Olson S","Alon S","Garrett SC"],"additional_accession":[]},"is_claimable":false,"name":"The majority of transcripts in the squid nervous system are extensively recoded by A-to-I RNA editing.","description":"RNA editing by adenosine deamination alters genetic information from the genomic blueprint. When it recodes mRNAs, it gives organisms the option to express diverse, functionally distinct, protein isoforms. All eumetazoans, from cnidarians to humans, express RNA editing enzymes. However, transcriptome-wide screens have only uncovered about 25 transcripts harboring conserved recoding RNA editing sites in mammals and several hundred recoding sites in Drosophila. These studies on few established models have led to the general assumption that recoding by RNA editing is extremely rare. Here we employ a novel bioinformatic approach with extensive validation to show that the squid Doryteuthis pealeii recodes proteins by RNA editing to an unprecedented extent. We identify 57,108 recoding sites in the nervous system, affecting the majority of the proteins studied. Recoding is tissue-dependent, and enriched in genes with neuronal and cytoskeletal functions, suggesting it plays an important role in brain physiology.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jan","modification":"2024-11-12T05:22:39.612Z","creation":"2019-03-27T01:49:20Z"},"accession":"S-EPMC4384741","cross_references":{"pubmed":["25569156"],"doi":["10.7554/eLife.05198"]}}