biostudies-literatureMarkov DNIGMS NIH HHS4628-37https://www.ebi.ac.uk/biostudies/studies/S-EPMC438596biostudies-literatureUnknown186(14)Escherichia coli strain 397c carries a temperature-sensitive mutation, rpoC397, that removes the last 50 amino acids of the RNA polymerase beta' subunit and is nonpermissive for plating of bacteriophage P2. P2 gor mutants productively infect 397c and define a new gene, lysC, encoded by a reading frame that extensively overlaps the P2 lysis accessory gene, lysB. The unusual location of lysC with respect to lysB is reminiscent of the Rz/Rz1 lysis gene pair of phage lambda. Indeed, coexpression of lysB and lysC complemented the growth defect of lambda Rz/Rz1 null mutants, indicating that the LysB/C pair is similar to Rz/Rz1 in both gene arrangement and function. Cells carrying the rpoC397 mutation exhibited an early onset of P2-induced lysis, which was suppressed by the gor mutation in lysC. Journal of bacteriologyP2 growth restriction on an rpoC mutant is suppressed by alleles of the Rz1 homolog lysC.PMC438596GM59295R01 GM027099R37 GM027099R01 GM059295GM27099Young RSauer BPark TSeverinov KMarkov DChristie GECalendar RfalseP2 growth restriction on an rpoC mutant is suppressed by alleles of the Rz1 homolog lysC.Escherichia coli strain 397c carries a temperature-sensitive mutation, rpoC397, that removes the last 50 amino acids of the RNA polymerase beta' subunit and is nonpermissive for plating of bacteriophage P2. P2 gor mutants productively infect 397c and define a new gene, lysC, encoded by a reading frame that extensively overlaps the P2 lysis accessory gene, lysB. The unusual location of lysC with respect to lysB is reminiscent of the Rz/Rz1 lysis gene pair of phage lambda. Indeed, coexpression of lysB and lysC complemented the growth defect of lambda Rz/Rz1 null mutants, indicating that the LysB/C pair is similar to Rz/Rz1 in both gene arrangement and function. Cells carrying the rpoC397 mutation exhibited an early onset of P2-induced lysis, which was suppressed by the gor mutation in lysC. 2004-01-01T00:00:00Z2004 Jul2025-04-04T21:19:03.834Z2019-03-27T00:50:33ZS-EPMC4385961523179610.1128/jb.186.14.4628-4637.200410.1128/JB.186.14.4628-4637.2004