biostudies-literatureThimgan MSNIDDK NIH HHSNIA NIH HHSNCRR NIH HHSNINDS NIH HHS801-14https://www.ebi.ac.uk/biostudies/studies/S-EPMC4402663biostudies-literatureUnknown38(5)<h4>Background and study objectives</h4>Flies mutant for the canonical clock protein cycle (cyc(01)) exhibit a sleep rebound that is ∼10 times larger than wild-type flies and die after only 10 h of sleep deprivation. Surprisingly, when starved, cyc(01) mutants can remain awake for 28 h without demonstrating negative outcomes. Thus, we hypothesized that identifying transcripts that are differentially regulated between waking induced by sleep deprivation and waking induced by starvation would identify genes that underlie the deleterious effects of sleep deprivation and/or protect flies from the negative consequences of waking.<h4>Design</h4>We used partial complementary DNA microarrays to identify transcripts that are differentially expressed between cyc(01) mutants that had been sleep depriSleepIdentification of genes associated with resilience/vulnerability to sleep deprivation and starvation in Drosophila.PMC4402663P41-RR00954R37-DK34388P01 AG033561P30-DK56341P60-DK205791 P30 DK063491-03P30 DK0205792 R01 NS051305-09P30 DK56341R01 NS051305P30 DK056341Seugnet LShaw PJTurk JThimgan MSfalseIdentification of genes associated with resilience/vulnerability to sleep deprivation and starvation in Drosophila.<h4>Background and study objectives</h4>Flies mutant for the canonical clock protein cycle (cyc(01)) exhibit a sleep rebound that is ∼10 times larger than wild-type flies and die after only 10 h of sleep deprivation. Surprisingly, when starved, cyc(01) mutants can remain awake for 28 h without demonstrating negative outcomes. Thus, we hypothesized that identifying transcripts that are differentially regulated between waking induced by sleep deprivation and waking induced by starvation would identify genes that underlie the deleterious effects of sleep deprivation and/or protect flies from the negative consequences of waking.<h4>Design</h4>We used partial complementary DNA microarrays to identify transcripts that are differentially expressed between cyc(01) mutants that had been sleep depri2015-01-01T00:00:00Z2015 May2026-04-07T14:41:52.444Z2019-03-27T01:50:09ZS-EPMC44026632540910410.5665/sleep.4680