biostudies-literatureUnknown9(5)Kojima HLarge sulfur-oxidizing bacteria, which accumulate a high concentration of nitrate, are important constituents of aquatic sediment ecosystems. No representative of this group has been isolated in pure culture, and only fragmented draft genome sequences are available for these microorganisms. In this study, we successfully reconstituted the genome of Thioploca ingrica from metagenomic sequences, thereby generating the first complete genome sequence from this group. The Thioploca samples for the metagenomic analysis were obtained from a freshwater lake in Japan. A PCR-free paired-end library was constructed from the DNA extracted from the samples and was sequenced on the Illumina MiSeq platform. By closing gaps within and between the scaffolds, we obtained a circular chromosome and a plasmid-The ISME journal1166-76https://www.ebi.ac.uk/biostudies/studies/S-EPMC4409161biostudies-literatureEcophysiology of Thioploca ingrica as revealed by the complete genome sequence supplemented with proteomic evidence.PMC4409161Ogura YYamamoto NWatanabe TKojima HTogashi TMori HFukui MNemoto FHayashi TKurokawa KfalseEcophysiology of Thioploca ingrica as revealed by the complete genome sequence supplemented with proteomic evidence.Large sulfur-oxidizing bacteria, which accumulate a high concentration of nitrate, are important constituents of aquatic sediment ecosystems. No representative of this group has been isolated in pure culture, and only fragmented draft genome sequences are available for these microorganisms. In this study, we successfully reconstituted the genome of Thioploca ingrica from metagenomic sequences, thereby generating the first complete genome sequence from this group. The Thioploca samples for the metagenomic analysis were obtained from a freshwater lake in Japan. A PCR-free paired-end library was constructed from the DNA extracted from the samples and was sequenced on the Illumina MiSeq platform. By closing gaps within and between the scaffolds, we obtained a circular chromosome and a plasmid-2015-01-01T00:00:00Z2015 May2026-05-02T00:21:38.933Z2019-03-27T01:50:29ZS-EPMC44091612534351310.1038/ismej.2014.209