{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2015"],"submitter":["Frickmann H"],"funding":["Körber Foundation"],"pubmed_abstract":["<h4>Introduction</h4>The aim of the study was the evaluation of panfungal PCR protocols with subsequent sequence analysis for the diagnostic identification of invasive mycoses in formalin-fixed, paraffin-embedded tissue samples with rare tropical mycoses.<h4>Materials and methods</h4>Five different previously described panfungal PCR/sequencing protocols targeting 18S and 28S ribosomal RNA gene fragments as well as internal transcribed spacer 1 and 2 fragments were evaluated with a collection of 17 formalin-fixed, paraffin-embedded tissue samples of patients with rare and/or tropical invasive mycoses, comprising chromoblastomycosis, coccidioidomycosis, cryptococcosis, histoplasmosis, mucormycosis, mycetoma/maduromycosis, and rhinosporidiosis, in a proof-of-principle analysis.<h4>Results</h4"],"journal":["BioMed research international"],"pagination":["938721"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4417575"],"repository":["biostudies-literature"],"pubmed_title":["Detection of tropical fungi in formalin-fixed, paraffin-embedded tissue: still an indication for microscopy in times of sequence-based diagnosis?"],"pmcid":["PMC4417575"],"pubmed_authors":["Hagen RM","Racz P","Haeupler A","Eggert P","Frickmann H","Tenner-Racz K","Bialek R","Loderstaedt U"],"additional_accession":[]},"is_claimable":false,"name":"Detection of tropical fungi in formalin-fixed, paraffin-embedded tissue: still an indication for microscopy in times of sequence-based diagnosis?","description":"<h4>Introduction</h4>The aim of the study was the evaluation of panfungal PCR protocols with subsequent sequence analysis for the diagnostic identification of invasive mycoses in formalin-fixed, paraffin-embedded tissue samples with rare tropical mycoses.<h4>Materials and methods</h4>Five different previously described panfungal PCR/sequencing protocols targeting 18S and 28S ribosomal RNA gene fragments as well as internal transcribed spacer 1 and 2 fragments were evaluated with a collection of 17 formalin-fixed, paraffin-embedded tissue samples of patients with rare and/or tropical invasive mycoses, comprising chromoblastomycosis, coccidioidomycosis, cryptococcosis, histoplasmosis, mucormycosis, mycetoma/maduromycosis, and rhinosporidiosis, in a proof-of-principle analysis.<h4>Results</h4","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2025-04-18T15:05:45.128Z","creation":"2019-03-27T01:50:53Z"},"accession":"S-EPMC4417575","cross_references":{"pubmed":["25961048"],"doi":["10.1155/2015/938721"]}}