biostudies-literatureUnknown26(6)Patel EBiotechnology and Biological Sciences Research CouncilDespite recent improvements to in situ proteolysis strategies, a higher efficiency is still needed to increase both the number of peptides detected and the associated ion intensity, leading to a complete and reliable set of biomarkers for diagnostic or prognostic purposes. In the study presented here, an extract of a systematic study is illustrated investigating a range of surfactants assisting trypsin proteolytic activity. Method development was trialled on fingermarks; this specimen results from a transfer of sweat from an individual's fingertip to a surface upon contact. As sweat carries a plethora of biomolecules, including peptides and proteins, fingermarks are, potentially, a very valuable specimen for non-invasive prognostic or diagnostic screening. A recent study has demonstrated tJournal of the American Society for Mass Spectrometry862-72https://www.ebi.ac.uk/biostudies/studies/S-EPMC4422860biostudies-literatureAlternative surfactants for improved efficiency of in situ tryptic proteolysis of fingermarks.PMC4422860Francese SPatel EMarshall NWest AMarshall PSClench MRfalseAlternative surfactants for improved efficiency of in situ tryptic proteolysis of fingermarks.Despite recent improvements to in situ proteolysis strategies, a higher efficiency is still needed to increase both the number of peptides detected and the associated ion intensity, leading to a complete and reliable set of biomarkers for diagnostic or prognostic purposes. In the study presented here, an extract of a systematic study is illustrated investigating a range of surfactants assisting trypsin proteolytic activity. Method development was trialled on fingermarks; this specimen results from a transfer of sweat from an individual's fingertip to a surface upon contact. As sweat carries a plethora of biomolecules, including peptides and proteins, fingermarks are, potentially, a very valuable specimen for non-invasive prognostic or diagnostic screening. A recent study has demonstrated t2015-01-01T00:00:00Z2015 Jun2025-04-22T10:28:11.826Z2019-03-27T01:51:08ZS-EPMC44228602591659910.1007/s13361-015-1140-z