{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Santangelo PJ"],"funding":["NIBIB NIH HHS","NCRR NIH HHS","NIAID NIH HHS","NIH HHS"],"pagination":["427-32"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4425449"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(5)"],"pubmed_abstract":["The detection of viral dynamics and localization in the context of controlled HIV infection remains a challenge and is limited to blood and biopsies. We developed a method to capture total-body simian immunodeficiency virus (SIV) replication using immunoPET (antibody-targeted positron emission tomography). The administration of a poly(ethylene glycol)-modified, (64)Cu-labeled SIV Gp120-specific antibody led to readily detectable signals in the gastrointestinal and respiratory tract, lymphoid tissues and reproductive organs of viremic monkeys. Viral signals were reduced in aviremic antiretroviral-treated monkeys but detectable in colon, select lymph nodes, small bowel, nasal turbinates, the genital tract and lung. In elite controllers, virus was detected primarily in foci in the small bowel, select lymphoid areas and the male reproductive tract, as confirmed by quantitative reverse-transcription PCR (qRT-PCR) and immunohistochemistry. This real-time, in vivo viral imaging method has broad applications to the study of immunodeficiency virus pathogenesis, drug and vaccine development, and the potential for clinical translation."],"journal":["Nature methods"],"pubmed_title":["Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy-treated macaques."],"pmcid":["PMC4425449"],"funding_grant_id":["R21 AI095129","P51 OD011132","P51 RR000165","U19 AI096187","8R24 OD010947","P51 OD11132","P30 AI050409","R24 OD010947","R21-AI095129","T32 EB006343","P30 AI045008","R01 AI078773","R01 AI078775","R01 AI111907","R01 AI098628"],"pubmed_authors":["Gumber S","Strait K","Ansari AA","Amancha PK","Hong JJ","Hoxie JA","Blanchard EL","Vidakovic B","Villinger F","Hunter E","Schuster DM","Byrareddy SN","Santangelo PJ","Rogers KA","Zurla C","Connor-Stroud F"],"additional_accession":[]},"is_claimable":false,"name":"Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy-treated macaques.","description":"The detection of viral dynamics and localization in the context of controlled HIV infection remains a challenge and is limited to blood and biopsies. We developed a method to capture total-body simian immunodeficiency virus (SIV) replication using immunoPET (antibody-targeted positron emission tomography). The administration of a poly(ethylene glycol)-modified, (64)Cu-labeled SIV Gp120-specific antibody led to readily detectable signals in the gastrointestinal and respiratory tract, lymphoid tissues and reproductive organs of viremic monkeys. Viral signals were reduced in aviremic antiretroviral-treated monkeys but detectable in colon, select lymph nodes, small bowel, nasal turbinates, the genital tract and lung. In elite controllers, virus was detected primarily in foci in the small bowel, select lymphoid areas and the male reproductive tract, as confirmed by quantitative reverse-transcription PCR (qRT-PCR) and immunohistochemistry. This real-time, in vivo viral imaging method has broad applications to the study of immunodeficiency virus pathogenesis, drug and vaccine development, and the potential for clinical translation.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 May","modification":"2020-10-29T09:56:50Z","creation":"2019-03-27T01:51:16Z"},"accession":"S-EPMC4425449","cross_references":{"pubmed":["25751144"],"doi":["10.1038/nmeth.3320"]}}