{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stuckey R"],"funding":["Consejería Economía, Innovación, Ciencia y Empleo, Junta de Andalucia","Ministerio de Ciencia e Innovación"],"pagination":["5779-84"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4426422"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["112(18)"],"pubmed_abstract":["DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by DNA polymerase-α and its intrinsic RNA primase activity. Here, we describe a novel origin-independent replication process that is mediated by transcription. RNA polymerase I transcription constraints lead to persistent RNA:DNA hybrids (R-loops) that prime replication in the ribosomal DNA locus. Our results suggest that eukaryotic genomes have developed tools to prevent R-loop-mediated replication events that potentially contribute to copy number variation, particularly relevant to carcinogenesis."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Role for RNA:DNA hybrids in origin-independent replication priming in a eukaryotic system."],"pmcid":["PMC4426422"],"funding_grant_id":["BIO2006-08051","P09/CVI4567","P08-CTS-04297","BIO-026","BIO-102","P11-CTS-7962","BFU2010-21339","CSD2007-0015","BFU2010-16372"],"pubmed_authors":["Garcia-Rodriguez N","Wellinger RE","Aguilera A","Stuckey R"],"additional_accession":[]},"is_claimable":false,"name":"Role for RNA:DNA hybrids in origin-independent replication priming in a eukaryotic system.","description":"DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by DNA polymerase-α and its intrinsic RNA primase activity. Here, we describe a novel origin-independent replication process that is mediated by transcription. RNA polymerase I transcription constraints lead to persistent RNA:DNA hybrids (R-loops) that prime replication in the ribosomal DNA locus. Our results suggest that eukaryotic genomes have developed tools to prevent R-loop-mediated replication events that potentially contribute to copy number variation, particularly relevant to carcinogenesis.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 May","modification":"2025-04-04T07:30:29.565Z","creation":"2019-03-27T01:51:19Z"},"accession":"S-EPMC4426422","cross_references":{"pubmed":["25902524"],"doi":["10.1073/pnas.1501769112"]}}