biostudies-literatureSterpone FEuropean Research Council4871-93https://www.ebi.ac.uk/biostudies/studies/S-EPMC4426487biostudies-literatureUnknown43(13)The OPEP coarse-grained protein model has been applied to a wide range of applications since its first release 15 years ago. The model, which combines energetic and structural accuracy and chemical specificity, allows the study of single protein properties, DNA-RNA complexes, amyloid fibril formation and protein suspensions in a crowded environment. Here we first review the current state of the model and the most exciting applications using advanced conformational sampling methods. We then present the current limitations and a perspective on the ongoing developments.Chemical Society reviewsThe OPEP protein model: from single molecules, amyloid formation, crowding and hydrodynamics to DNA/RNA systems.PMC4426487258748St-Pierre JFLaurin YMousseau NChebaro YKalimeri MDerreumaux PCragnolini TPasquali STek ASterpone FTuffery PBaaden MMelchionna SNguyen PHBarducci AfalseThe OPEP protein model: from single molecules, amyloid formation, crowding and hydrodynamics to DNA/RNA systems.The OPEP coarse-grained protein model has been applied to a wide range of applications since its first release 15 years ago. The model, which combines energetic and structural accuracy and chemical specificity, allows the study of single protein properties, DNA-RNA complexes, amyloid fibril formation and protein suspensions in a crowded environment. Here we first review the current state of the model and the most exciting applications using advanced conformational sampling methods. We then present the current limitations and a perspective on the ongoing developments.2014-01-01T00:00:00Z2014 Jul2026-05-02T10:17:22.107Z2026-04-07T17:53:05.936ZS-EPMC44264872475993410.1039/c4cs00048j