biostudies-literatureYang LNICHD NIH HHSNHLBI NIH HHSe0126576https://www.ebi.ac.uk/biostudies/studies/S-EPMC4428707biostudies-literatureUnknown10(5)The surfactant protein (SP-A) receptor SP-R210 has been shown to increase phagocytosis of SP-A-bound pathogens and to modulate cytokine secretion by immune cells. SP-A plays an important role in pulmonary immunity by enhancing opsonization and clearance of pathogens and by modulating macrophage inflammatory responses. Alternative splicing of the Myo18A gene results in two isoforms: SP-R210S and SP-R210L, with the latter predominantly expressed in alveolar macrophages. In this study we show that SP-A is required for optimal expression of SP-R210L on alveolar macrophages. Interestingly, pre-treatment with SP-A prepared by different methods either enhances or suppresses responsiveness to LPS, possibly due to differential co-isolation of SP-B or other proteins. We also report that dominant negPloS oneSP-R210 (Myo18A) Isoforms as Intrinsic Modulators of Macrophage Priming and Activation.PMC4428707HL068127HL-34788K12 HD055882R01 HL034788R01 HL068127R37 HL034788Floros JHu SYang LSilveyra PDiAngelo SLHalstead ESChroneos ZCWu YMDavies MLChristensen NDUmstead TMCarrillo MMcCormack FXfalseSP-R210 (Myo18A) Isoforms as Intrinsic Modulators of Macrophage Priming and Activation.The surfactant protein (SP-A) receptor SP-R210 has been shown to increase phagocytosis of SP-A-bound pathogens and to modulate cytokine secretion by immune cells. SP-A plays an important role in pulmonary immunity by enhancing opsonization and clearance of pathogens and by modulating macrophage inflammatory responses. Alternative splicing of the Myo18A gene results in two isoforms: SP-R210S and SP-R210L, with the latter predominantly expressed in alveolar macrophages. In this study we show that SP-A is required for optimal expression of SP-R210L on alveolar macrophages. Interestingly, pre-treatment with SP-A prepared by different methods either enhances or suppresses responsiveness to LPS, possibly due to differential co-isolation of SP-B or other proteins. We also report that dominant neg2015-01-01T00:00:00Z20152025-04-22T07:09:00.803Z2019-03-26T23:32:41ZS-EPMC44287072596534610.1371/journal.pone.0126576