biostudies-literatureChan CEFIC NIH HHS10281https://www.ebi.ac.uk/biostudies/studies/S-EPMC4432570biostudies-literatureUnknown5The current clinical management of TB is complicated by the lack of suitable diagnostic tests that can be employed in infrastructure and resource poor regions. The mannose-capped form of lipoarabinomannan (ManLAM) is unique to the surface envelope of slow-growing, pathogenic mycobacteria such as M.tuberculosis (M.tb) and facilitates passive invasion of mononuclear phagocytes. The detection of this virulence factor in urine, sputum and serum has engendered interest in its employment as a biomarker for M.tb infection. In this study, we utilize a subtractive screening methodology to engineer the first high affinity recombinant antibody (My2F12) with exquisite specificity for the α1-2 mannose linkages enriched in ManLAM from M.tb. My2F12 binds to pathogenic mycobacterial species but not fast gScientific reportsThe diagnostic targeting of a carbohydrate virulence factor from M.Tuberculosis.PMC4432570D43 TW007124Seah GTHanson BJMacAry PAChan CESeeberger PHGotze STukvadze NWenk MRfalseThe diagnostic targeting of a carbohydrate virulence factor from M.Tuberculosis.The current clinical management of TB is complicated by the lack of suitable diagnostic tests that can be employed in infrastructure and resource poor regions. The mannose-capped form of lipoarabinomannan (ManLAM) is unique to the surface envelope of slow-growing, pathogenic mycobacteria such as M.tuberculosis (M.tb) and facilitates passive invasion of mononuclear phagocytes. The detection of this virulence factor in urine, sputum and serum has engendered interest in its employment as a biomarker for M.tb infection. In this study, we utilize a subtractive screening methodology to engineer the first high affinity recombinant antibody (My2F12) with exquisite specificity for the α1-2 mannose linkages enriched in ManLAM from M.tb. My2F12 binds to pathogenic mycobacterial species but not fast g2015-01-01T00:00:00Z2015 May2025-04-22T10:35:38.921Z2019-03-27T01:51:39ZS-EPMC44325702597587310.1038/srep10281