{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8"],"submitter":["Lieskovska J"],"pubmed_abstract":["Background
Transmission of pathogens by ticks is greatly supported by tick saliva released during feeding. Dendritic cells (DC) act as immunological sentinels and interconnect the innate and adaptive immune system. They control polarization of the immune response towards Th1 or Th2 phenotype. We investigated whether salivary cystatins from the hard tick Ixodes scapularis, sialostatin L (Sialo L) and sialostatin L2 (Sialo L2), influence mouse dendritic cells exposed to Borrelia burgdorferi and relevant Toll-like receptor ligands.Methods
DCs derived from bone-marrow by GM-CSF or Flt-3 ligand, were activated with Borrelia spirochetes or TLR ligands in the presence of 3 μM Sialo L and 3 μM Sialo L2. Produced chemokines and IFN-β were measured by ELISA test. The activation of si"],"journal":["Parasites & vectors"],"pagination":["275"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4436792"],"repository":["biostudies-literature"],"pubmed_title":["Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes."],"pmcid":["PMC4436792"],"pubmed_authors":["Langhansova H","Kopecky J","Lieskovska J","Campos Chagas A","Calvo E","Palenikova J","Kotsyfakis M"],"additional_accession":[]},"is_claimable":false,"name":"Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes.","description":"Background
Transmission of pathogens by ticks is greatly supported by tick saliva released during feeding. Dendritic cells (DC) act as immunological sentinels and interconnect the innate and adaptive immune system. They control polarization of the immune response towards Th1 or Th2 phenotype. We investigated whether salivary cystatins from the hard tick Ixodes scapularis, sialostatin L (Sialo L) and sialostatin L2 (Sialo L2), influence mouse dendritic cells exposed to Borrelia burgdorferi and relevant Toll-like receptor ligands.Methods
DCs derived from bone-marrow by GM-CSF or Flt-3 ligand, were activated with Borrelia spirochetes or TLR ligands in the presence of 3 μM Sialo L and 3 μM Sialo L2. Produced chemokines and IFN-β were measured by ELISA test. The activation of si","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 May","modification":"2025-04-04T07:29:36.919Z","creation":"2019-03-27T01:51:50Z"},"accession":"S-EPMC4436792","cross_references":{"pubmed":["25975355"],"doi":["10.1186/s13071-015-0887-1"]}}