biostudies-literatureUnknown26(10)Umeda KPrecise wiring patterns of axons are among the remarkable features of neuronal circuit formation, and establishment of the proper neuronal network requires control of outgrowth, branching, and guidance of axons. R-Ras is a Ras-family small GTPase that has essential roles in multiple phases of axonal development. We recently identified afadin, an F-actin-binding protein, as an effector of R-Ras mediating axon branching through F-actin reorganization. Afadin comprises two isoforms--l-afadin, having the F-actin-binding domain, and s-afadin, lacking the F-actin-binding domain. Compared with l-afadin, s-afadin, the short splicing variant of l-afadin, contains RA domains but lacks the F-actin-binding domain. Neurons express both isoforms; however, the function of s-afadin in brain remains unknowMolecular biology of the cell1957-70https://www.ebi.ac.uk/biostudies/studies/S-EPMC4436838biostudies-literatureA short splicing isoform of afadin suppresses the cortical axon branching in a dominant-negative manner.PMC4436838Umeda KNegishi MOinuma IIwasawa NfalseA short splicing isoform of afadin suppresses the cortical axon branching in a dominant-negative manner.Precise wiring patterns of axons are among the remarkable features of neuronal circuit formation, and establishment of the proper neuronal network requires control of outgrowth, branching, and guidance of axons. R-Ras is a Ras-family small GTPase that has essential roles in multiple phases of axonal development. We recently identified afadin, an F-actin-binding protein, as an effector of R-Ras mediating axon branching through F-actin reorganization. Afadin comprises two isoforms--l-afadin, having the F-actin-binding domain, and s-afadin, lacking the F-actin-binding domain. Compared with l-afadin, s-afadin, the short splicing variant of l-afadin, contains RA domains but lacks the F-actin-binding domain. Neurons express both isoforms; however, the function of s-afadin in brain remains unknow2015-01-01T00:00:00Z2015 May2026-04-29T07:42:07.427Z2019-03-27T01:51:51ZS-EPMC44368382580848910.1091/mbc.E15-01-0039