biostudies-literatureUnknown16Loux V<h4>Background</h4>Propionibacterium freudenreichii (PF) is an actinobacterium used in cheese technology and for its probiotic properties. PF is also extremely adaptable to several ecological niches and can grow on a variety of carbon and nitrogen sources. The aim of this work was to discover the genetic basis for strain-dependent traits related to its ability to use specific carbon sources. High-throughput sequencing technologies were ideal for this purpose as they have the potential to decipher genomic diversity at a moderate cost.<h4>Results</h4>21 strains of PF were sequenced and the genomes were assembled de novo. Scaffolds were ordered by comparison with the complete reference genome CIRM-BIA1, obtained previously using traditional Sanger sequencing. Automatic functional annotation aBMC genomics296https://www.ebi.ac.uk/biostudies/studies/S-EPMC4437456biostudies-literatureMutations and genomic islands can explain the strain dependency of sugar utilization in 21 strains of Propionibacterium freudenreichii.PMC4437456Almeida SParayre SLe Loir YChuat VBuratti JPeterlongo PAzevedo VHammani AFalentin HChiapello HMadec MNGendrault AAury JMMariadassou MBarbe VDeutsch SMJan GLoux VfalseMutations and genomic islands can explain the strain dependency of sugar utilization in 21 strains of Propionibacterium freudenreichii.<h4>Background</h4>Propionibacterium freudenreichii (PF) is an actinobacterium used in cheese technology and for its probiotic properties. PF is also extremely adaptable to several ecological niches and can grow on a variety of carbon and nitrogen sources. The aim of this work was to discover the genetic basis for strain-dependent traits related to its ability to use specific carbon sources. High-throughput sequencing technologies were ideal for this purpose as they have the potential to decipher genomic diversity at a moderate cost.<h4>Results</h4>21 strains of PF were sequenced and the genomes were assembled de novo. Scaffolds were ordered by comparison with the complete reference genome CIRM-BIA1, obtained previously using traditional Sanger sequencing. Automatic functional annotation a2015-01-01T00:00:00Z2015 Apr2026-06-13T05:53:18.922Z2019-03-27T01:51:52ZS-EPMC44374562588652210.1186/s12864-015-1467-7