{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(5)"],"submitter":["Harter PN"],"pubmed_abstract":["<h4>Background</h4>Current pathological diagnostics include the analysis of (epi-)genetic alterations as well as oncogenic pathways. Deregulated mammalian target of rapamycin complex 1 (mTORC1) signaling has been implicated in a variety of cancers including malignant gliomas and is considered a promising target in cancer treatment. Monitoring of mTORC1 activity before and during inhibitor therapy is essential. The aim of our study is to provide a recommendation and report on pitfalls in the use of phospho-specific antibodies against mTORC1-targets phospho-RPS6 (Ser235/236; Ser240/244) and phospho-4EBP1 (Thr37/46) in formalin fixed, paraffin embedded material.<h4>Methods and findings</h4>Primary, established cell lines and brain tumor tissue from routine diagnostics were assessed by immunoc"],"journal":["PloS one"],"pagination":["e0127123"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4437987"],"repository":["biostudies-literature"],"pubmed_title":["Immunohistochemical Assessment of Phosphorylated mTORC1-Pathway Proteins in Human Brain Tumors."],"pmcid":["PMC4437987"],"pubmed_authors":["Senft C","Ronellenfitsch MW","Ilina E","Baumgarten P","Tichy J","Thiepold AL","Steinbach JP","Zornig M","Burger MC","Harter PN","Jennewein L","Mittelbronn M"],"additional_accession":[]},"is_claimable":false,"name":"Immunohistochemical Assessment of Phosphorylated mTORC1-Pathway Proteins in Human Brain Tumors.","description":"<h4>Background</h4>Current pathological diagnostics include the analysis of (epi-)genetic alterations as well as oncogenic pathways. Deregulated mammalian target of rapamycin complex 1 (mTORC1) signaling has been implicated in a variety of cancers including malignant gliomas and is considered a promising target in cancer treatment. Monitoring of mTORC1 activity before and during inhibitor therapy is essential. The aim of our study is to provide a recommendation and report on pitfalls in the use of phospho-specific antibodies against mTORC1-targets phospho-RPS6 (Ser235/236; Ser240/244) and phospho-4EBP1 (Thr37/46) in formalin fixed, paraffin embedded material.<h4>Methods and findings</h4>Primary, established cell lines and brain tumor tissue from routine diagnostics were assessed by immunoc","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2026-04-19T03:22:58.488Z","creation":"2019-03-26T23:32:50Z"},"accession":"S-EPMC4437987","cross_references":{"pubmed":["25993328"],"doi":["10.1371/journal.pone.0127123"]}}