biostudies-literatureUnknown10(5)Harter PN<h4>Background</h4>Current pathological diagnostics include the analysis of (epi-)genetic alterations as well as oncogenic pathways. Deregulated mammalian target of rapamycin complex 1 (mTORC1) signaling has been implicated in a variety of cancers including malignant gliomas and is considered a promising target in cancer treatment. Monitoring of mTORC1 activity before and during inhibitor therapy is essential. The aim of our study is to provide a recommendation and report on pitfalls in the use of phospho-specific antibodies against mTORC1-targets phospho-RPS6 (Ser235/236; Ser240/244) and phospho-4EBP1 (Thr37/46) in formalin fixed, paraffin embedded material.<h4>Methods and findings</h4>Primary, established cell lines and brain tumor tissue from routine diagnostics were assessed by immunocPloS onee0127123https://www.ebi.ac.uk/biostudies/studies/S-EPMC4437987biostudies-literatureImmunohistochemical Assessment of Phosphorylated mTORC1-Pathway Proteins in Human Brain Tumors.PMC4437987Senft CRonellenfitsch MWIlina EBaumgarten PTichy JThiepold ALSteinbach JPZornig MBurger MCHarter PNJennewein LMittelbronn MfalseImmunohistochemical Assessment of Phosphorylated mTORC1-Pathway Proteins in Human Brain Tumors.<h4>Background</h4>Current pathological diagnostics include the analysis of (epi-)genetic alterations as well as oncogenic pathways. Deregulated mammalian target of rapamycin complex 1 (mTORC1) signaling has been implicated in a variety of cancers including malignant gliomas and is considered a promising target in cancer treatment. Monitoring of mTORC1 activity before and during inhibitor therapy is essential. The aim of our study is to provide a recommendation and report on pitfalls in the use of phospho-specific antibodies against mTORC1-targets phospho-RPS6 (Ser235/236; Ser240/244) and phospho-4EBP1 (Thr37/46) in formalin fixed, paraffin embedded material.<h4>Methods and findings</h4>Primary, established cell lines and brain tumor tissue from routine diagnostics were assessed by immunoc2015-01-01T00:00:00Z20152026-04-19T03:22:58.488Z2019-03-26T23:32:50ZS-EPMC44379872599332810.1371/journal.pone.0127123