{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Allam A"],"funding":["NIAID NIH HHS"],"pagination":["10443"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4451806"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5"],"pubmed_abstract":["CD4(+) T follicular helper cells (TFH) in germinal centers are required for maturation of B-cells. While the role of TFH-cells has been studied in blood and lymph nodes of HIV-1 infected individuals, its role in the mucosal tissues has not been investigated. We show that the gut and female reproductive tract (FRT) of humanized DRAG mice have a high level of human lymphocytes and a high frequency of TFH (CXCR5(+)PD-1(++)) and precursor-TFH (CXCR5(+)PD-1(+)) cells. The majority of TFH-cells expressed CCR5 and CXCR3 and are the most permissive to HIV-1 infection. A single low-dose intravaginal HIV-1 challenge of humanized DRAG mice results in 100% infectivity with accumulation of TFH-cells mainly in the Peyer's patches and FRT. The novel finding of TFH-cells in the FRT may contribute to the high susceptibility of DRAG mice to HIV-1 infection. This mouse model thus provides new opportunities to study TFH-cells and to evaluate HIV-1 vaccines."],"journal":["Scientific reports"],"pubmed_title":["TFH cells accumulate in mucosal tissues of humanized-DRAG mice and are highly permissive to HIV-1."],"pmcid":["PMC4451806"],"funding_grant_id":["AI102725","R01 AI102725"],"pubmed_authors":["Jagodzinski L","Casares S","Majji S","Peachman K","Kim J","Allam A","Alving CR","Merbah M","Wijayalath W","Ratto-Kim S","Kim JH","Michael NL","Rao M"],"additional_accession":[]},"is_claimable":false,"name":"TFH cells accumulate in mucosal tissues of humanized-DRAG mice and are highly permissive to HIV-1.","description":"CD4(+) T follicular helper cells (TFH) in germinal centers are required for maturation of B-cells. While the role of TFH-cells has been studied in blood and lymph nodes of HIV-1 infected individuals, its role in the mucosal tissues has not been investigated. We show that the gut and female reproductive tract (FRT) of humanized DRAG mice have a high level of human lymphocytes and a high frequency of TFH (CXCR5(+)PD-1(++)) and precursor-TFH (CXCR5(+)PD-1(+)) cells. The majority of TFH-cells expressed CCR5 and CXCR3 and are the most permissive to HIV-1 infection. A single low-dose intravaginal HIV-1 challenge of humanized DRAG mice results in 100% infectivity with accumulation of TFH-cells mainly in the Peyer's patches and FRT. The novel finding of TFH-cells in the FRT may contribute to the high susceptibility of DRAG mice to HIV-1 infection. This mouse model thus provides new opportunities to study TFH-cells and to evaluate HIV-1 vaccines.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jun","modification":"2025-04-26T08:00:29.522Z","creation":"2019-03-27T01:52:35Z"},"accession":"S-EPMC4451806","cross_references":{"pubmed":["26034905"],"doi":["10.1038/srep10443"]}}