biostudies-literatureUnknown5Hsu CHmib(nn2002), found from an allele screen, showed early segmentation defect and severe cell death phenotypes, which are different from previously known mib mutants. Despite distinct morphological phenotypes, the typical mib molecular phenotypes: her4 down-regulation, neurogenic phenotype and cold sensitive dlc expression pattern, still remained. The linkage analysis also indicated that mib(nn2002) is a new mib allele. Failure of specification in anterior 7-10 somites is likely due to lack of foxc1a expression in mib(nn2002) homozygotes. Somites and somite markers gradually appeared after 7-10 somite stage, suggesting that foxc1a is only essential for the formation of anterior 7-10 somites. Apoptosis began around 16-somite stage with p53 up-regulation. To find the possible links of mib, foxcScientific reports10673https://www.ebi.ac.uk/biostudies/studies/S-EPMC4454137biostudies-literatureA new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.PMC4454137Lin JSChan TFTse WKYou MSPo Lai KHsu CHJiang YJLi JWfalseA new mib allele with a chromosomal deletion covering foxc1a exhibits anterior somite specification defect.mib(nn2002), found from an allele screen, showed early segmentation defect and severe cell death phenotypes, which are different from previously known mib mutants. Despite distinct morphological phenotypes, the typical mib molecular phenotypes: her4 down-regulation, neurogenic phenotype and cold sensitive dlc expression pattern, still remained. The linkage analysis also indicated that mib(nn2002) is a new mib allele. Failure of specification in anterior 7-10 somites is likely due to lack of foxc1a expression in mib(nn2002) homozygotes. Somites and somite markers gradually appeared after 7-10 somite stage, suggesting that foxc1a is only essential for the formation of anterior 7-10 somites. Apoptosis began around 16-somite stage with p53 up-regulation. To find the possible links of mib, foxc2015-01-01T00:00:00Z2015 Jun2026-05-04T05:30:05.203Z2019-03-27T01:52:43ZS-EPMC44541372603989410.1038/srep10673