{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Poposki JA"],"funding":["NICHD NIH HHS","NIAID NIH HHS","NHLBI NIH HHS"],"pagination":["384-93"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4467201"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["45(2)"],"pubmed_abstract":["Although chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2 inflammation, the mechanism underlying the onset and amplification of this inflammation has not been fully elucidated. Dendritic cells (DCs) are major antigen-presenting cells, central inducers of adaptive immunity and critical regulators of many inflammatory diseases. However, the presence of DCs in CRS, especially in nasal polyps (NPs), has not been extensively studied.The objective of this study was to characterize DC subsets in CRS.We used real-time PCR to assess the expression of mRNA for markers of myeloid DCs (mDCs; CD1c), plasmacytoid DCs (pDCs; CD303) and Langerhans cells (LCs; CD1a, CD207) in uncinate tissue (UT) from controls and patients with CRS as well as in NP. We assayed the presence of DCs by immunohistochemistry and flow cytometry.Compared to UT from control subjects (n = 15) and patients with CRS without NP (CRSsNP) (n = 16) and CRSwNP (n = 17), mRNAs for CD1a and CD1c were significantly elevated in NPs (n = 29). In contrast, CD207 mRNA was not elevated in NPs. Immunohistochemistry showed that CD1c(+) cells but not CD303(+) cells were significantly elevated in NPs compared to control subjects or patients with CRSsNP. Flow cytometric analysis showed that CD1a(+) cells in NPs might be a subset of mDC1s and that CD45(+) CD19(-) CD1c(+) CD11c(+) CD141(-) CD303(-) HLA-DR(+) mDC1s and CD45(+) CD19(-) CD11c(+) CD1c(-) CD141(high) HLA-DR(+) mDC2s were significantly elevated in NPs compared to UT from controls and CRSsNP, but CD45(+) CD11c(-) CD303(+) HLA-DR(+) pDCs were only elevated in NPs compared to control UT.Myeloid DCs are elevated in CRSwNP, especially in NPs. Myeloid DCs thus may indirectly contribute to the inflammation observed in CRSwNP."],"journal":["Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology"],"pubmed_title":["Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis."],"pmcid":["PMC4467201"],"funding_grant_id":["K12 HD055884","R01 AI104733","R37 HL068546","U19 AI106683","R21 HL113913","R01 HL078860"],"pubmed_authors":["Grammer LC","Norton J","Chandra RK","Tan BK","Harris KE","Conley DB","Poposki JA","Peters AT","Suh LA","Hulse KE","Welch K","Peterson S","Carter R","Kato A","Schleimer RP","Kern RC"],"additional_accession":[]},"is_claimable":false,"name":"Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis.","description":"Although chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2 inflammation, the mechanism underlying the onset and amplification of this inflammation has not been fully elucidated. Dendritic cells (DCs) are major antigen-presenting cells, central inducers of adaptive immunity and critical regulators of many inflammatory diseases. However, the presence of DCs in CRS, especially in nasal polyps (NPs), has not been extensively studied.The objective of this study was to characterize DC subsets in CRS.We used real-time PCR to assess the expression of mRNA for markers of myeloid DCs (mDCs; CD1c), plasmacytoid DCs (pDCs; CD303) and Langerhans cells (LCs; CD1a, CD207) in uncinate tissue (UT) from controls and patients with CRS as well as in NP. We assayed the presence of DCs by immunohistochemistry and flow cytometry.Compared to UT from control subjects (n = 15) and patients with CRS without NP (CRSsNP) (n = 16) and CRSwNP (n = 17), mRNAs for CD1a and CD1c were significantly elevated in NPs (n = 29). In contrast, CD207 mRNA was not elevated in NPs. Immunohistochemistry showed that CD1c(+) cells but not CD303(+) cells were significantly elevated in NPs compared to control subjects or patients with CRSsNP. Flow cytometric analysis showed that CD1a(+) cells in NPs might be a subset of mDC1s and that CD45(+) CD19(-) CD1c(+) CD11c(+) CD141(-) CD303(-) HLA-DR(+) mDC1s and CD45(+) CD19(-) CD11c(+) CD1c(-) CD141(high) HLA-DR(+) mDC2s were significantly elevated in NPs compared to UT from controls and CRSsNP, but CD45(+) CD11c(-) CD303(+) HLA-DR(+) pDCs were only elevated in NPs compared to control UT.Myeloid DCs are elevated in CRSwNP, especially in NPs. Myeloid DCs thus may indirectly contribute to the inflammation observed in CRSwNP.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Feb","modification":"2020-10-31T08:45:12Z","creation":"2019-03-27T01:53:27Z"},"accession":"S-EPMC4467201","cross_references":{"pubmed":["25469646"],"doi":["10.1111/cea.12471"]}}