biostudies-literatureShanks RMNEI NIH HHSNIDDK NIH HHSNIAID NIH HHSNCI NIH HHS2907-16https://www.ebi.ac.uk/biostudies/studies/S-EPMC4468537biostudies-literatureUnknown83(7)The Gram-negative bacterium and opportunistic pathogen Serratia marcescens causes ocular infections in healthy individuals. Secreted protease activity was characterized from 44 ocular clinical isolates, and a higher frequency of protease-positive strains was observed among keratitis isolates than among conjunctivitis isolates. A positive correlation between protease activity and cytotoxicity to human corneal epithelial cells in vitro was determined. Deletion of prtS in clinical keratitis isolate K904 reduced, but did not eliminate, cytotoxicity and secreted protease production. This indicated that PrtS is necessary for full cytotoxicity to ocular cells and implied the existence of another secreted protease(s) and cytotoxic factors. Bioinformatic analysis of the S. marcescens Db11 genome reInfection and immunityIdentification of SlpB, a Cytotoxic Protease from Serratia marcescens.PMC4468537P30 EY085570AI085570DK083284P30 CA047904R56 DK083284P30CA047904T32 EY0172712T32EY017271R01 AI085570R01 DK083284Stella NAHunt KMBrothers KMShanks RMZhang LThibodeau PHfalseIdentification of SlpB, a Cytotoxic Protease from Serratia marcescens.The Gram-negative bacterium and opportunistic pathogen Serratia marcescens causes ocular infections in healthy individuals. Secreted protease activity was characterized from 44 ocular clinical isolates, and a higher frequency of protease-positive strains was observed among keratitis isolates than among conjunctivitis isolates. A positive correlation between protease activity and cytotoxicity to human corneal epithelial cells in vitro was determined. Deletion of prtS in clinical keratitis isolate K904 reduced, but did not eliminate, cytotoxicity and secreted protease production. This indicated that PrtS is necessary for full cytotoxicity to ocular cells and implied the existence of another secreted protease(s) and cytotoxic factors. Bioinformatic analysis of the S. marcescens Db11 genome re2015-01-01T00:00:00Z2015 Jul2025-04-19T05:40:04.995Z2019-03-27T01:53:29ZS-EPMC44685372593950910.1128/iai.03096-1410.1128/IAI.03096-14