biostudies-literatureYuan SNICHD NIH HHSNIDDK NIH HHSNHLBI NIH HHS556-67https://www.ebi.ac.uk/biostudies/studies/S-EPMC4469357biostudies-literatureUnknown25(5)<h4>Background</h4>Bilateral symmetry during vertebrate development is broken at the left-right organizer (LRO) by ciliary motility and the resultant directional flow of extracellular fluid. However, how ciliary motility is perceived and transduced into asymmetrical intracellular signaling at the LRO remains controversial. Previous work has indicated that sensory cilia and polycystin-2 (Pkd2), a cation channel, are required for sensing ciliary motility, yet their function and the molecular mechanism linking both to left-right signaling cascades are unknown.<h4>Results</h4>Here we report novel intraciliary calcium oscillations (ICOs) at the LRO that connect ciliary sensation of ciliary motility to downstream left-right signaling. Utilizing cilia-targeted genetically encoded calcium indicatoCurrent biology : CBIntraciliary calcium oscillations initiate vertebrate left-right asymmetry.PMC4469357R01 DK0928081P30DK090744-01R01 HL093280R01HL093280P30 DK090744R01 HL1258855T32HD00709436R01DK092808-01A1T32 HD007094Yuan SZhao LBrueckner MSun ZfalseIntraciliary calcium oscillations initiate vertebrate left-right asymmetry.<h4>Background</h4>Bilateral symmetry during vertebrate development is broken at the left-right organizer (LRO) by ciliary motility and the resultant directional flow of extracellular fluid. However, how ciliary motility is perceived and transduced into asymmetrical intracellular signaling at the LRO remains controversial. Previous work has indicated that sensory cilia and polycystin-2 (Pkd2), a cation channel, are required for sensing ciliary motility, yet their function and the molecular mechanism linking both to left-right signaling cascades are unknown.<h4>Results</h4>Here we report novel intraciliary calcium oscillations (ICOs) at the LRO that connect ciliary sensation of ciliary motility to downstream left-right signaling. Utilizing cilia-targeted genetically encoded calcium indicato2015-01-01T00:00:00Z2015 Mar2025-05-18T12:29:30.839Z2025-05-18T12:29:30.839ZS-EPMC44693572566053910.1016/j.cub.2014.12.051