{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(6)"],"submitter":["Yoon S"],"pubmed_abstract":["Adaptive immunity in homeotherms depends greatly on CD4+ Th cells which release cytokines in response to specific antigen stimulation. Whilst bony fish and poikilothermic tetrapods possess cells that express TcR and CD4-related genes (that exist in two forms in teleost fish; termed CD4-1 and CD4-2), to date there is no unequivocal demonstration that cells equivalent to Th exist. Thus, in this study we determined whether CD4-1+ lymphocytes can express cytokines typical of Th cells following antigen specific stimulation, using the zebrafish (Danio rerio). Initially, we analyzed the CD4 locus in zebrafish and found three CD4 homologues, a CD4-1 molecule and two CD4-2 molecules. The zfCD4-1 and zfCD4-2 transcripts were detected in immune organs and were most highly expressed in lymphocytes. A "],"journal":["PloS one"],"pagination":["e0126378"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4470515"],"repository":["biostudies-literature"],"pubmed_title":["First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)."],"pmcid":["PMC4470515"],"pubmed_authors":["Bird S","Weerdenburg EM","Zou J","van der Sar AM","Wyse C","Yoon S","Wang D","Secombes CJ","Alnabulsi A","Mitra S"],"additional_accession":[]},"is_claimable":false,"name":"First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio).","description":"Adaptive immunity in homeotherms depends greatly on CD4+ Th cells which release cytokines in response to specific antigen stimulation. Whilst bony fish and poikilothermic tetrapods possess cells that express TcR and CD4-related genes (that exist in two forms in teleost fish; termed CD4-1 and CD4-2), to date there is no unequivocal demonstration that cells equivalent to Th exist. Thus, in this study we determined whether CD4-1+ lymphocytes can express cytokines typical of Th cells following antigen specific stimulation, using the zebrafish (Danio rerio). Initially, we analyzed the CD4 locus in zebrafish and found three CD4 homologues, a CD4-1 molecule and two CD4-2 molecules. The zfCD4-1 and zfCD4-2 transcripts were detected in immune organs and were most highly expressed in lymphocytes. A ","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2026-06-02T16:17:46.271Z","creation":"2019-03-26T23:33:18Z"},"accession":"S-EPMC4470515","cross_references":{"pubmed":["26083432"],"doi":["10.1371/journal.pone.0126378"]}}