{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["34(6)"],"submitter":["Wang S"],"pubmed_abstract":["Viral factor has been implicated in the etiopathogenesis of vitiligo. To elucidate the effects of viral double-stranded RNA (dsRNA) on melanocytes and to explore the underlying mechanisms, primary cultured normal human melanocytes were treated with synthetic viral dsRNA analog poly(I:C). The results demonstrated that poly(I:C)-triggered apoptosis when transfected into melanocytes, while extracellular poly(I:C) did not have that effect. Intracellular poly(I:C)-induced melanocyte death was decreased by RIG-I or MDA5 siRNA, but not by TLR3 siRNA. Both intracellular and extracellular poly(I:C) induced the expression of IFNB, TNF, IL6, and IL8. However, extracellular poly(I:C) demonstrated a much weaker induction capacity of cytokine genes than intracellular poly(I:C). Further analysis revealed"],"journal":["DNA and cell biology"],"pagination":["391-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4485883"],"repository":["biostudies-literature"],"pubmed_title":["Differential responses of normal human melanocytes to intra- and extracellular dsRNA."],"pmcid":["PMC4485883"],"pubmed_authors":["Jin R","Zhu Y","Wang S","Xu A","Liu D"],"additional_accession":[]},"is_claimable":false,"name":"Differential responses of normal human melanocytes to intra- and extracellular dsRNA.","description":"Viral factor has been implicated in the etiopathogenesis of vitiligo. To elucidate the effects of viral double-stranded RNA (dsRNA) on melanocytes and to explore the underlying mechanisms, primary cultured normal human melanocytes were treated with synthetic viral dsRNA analog poly(I:C). The results demonstrated that poly(I:C)-triggered apoptosis when transfected into melanocytes, while extracellular poly(I:C) did not have that effect. Intracellular poly(I:C)-induced melanocyte death was decreased by RIG-I or MDA5 siRNA, but not by TLR3 siRNA. Both intracellular and extracellular poly(I:C) induced the expression of IFNB, TNF, IL6, and IL8. However, extracellular poly(I:C) demonstrated a much weaker induction capacity of cytokine genes than intracellular poly(I:C). Further analysis revealed","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jun","modification":"2025-04-04T12:51:41.772Z","creation":"2019-03-27T01:54:16Z"},"accession":"S-EPMC4485883","cross_references":{"pubmed":["25803620"],"doi":["10.1089/dna.2014.2711"]}}