{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hesketh EE"],"funding":["Kidney Research UK"],"pagination":["e0131849"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4488261"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(6)"],"pubmed_abstract":["Autoimmunity is associated with defective phagocytic clearance of apoptotic cells. IgM deficient mice exhibit an autoimmune phenotype consistent with a role for circulating IgM antibodies in apoptotic cell clearance. We have extensively characterised IgM binding to non-apoptotic and apoptotic mouse thymocytes and human Jurkat cells using flow cytometry, confocal imaging and electron microscopy. We demonstrate strong specific IgM binding to a subset of Annexin-V (AnnV)+PI (Propidium Iodide)+ apoptotic cells with disrupted cell membranes. Electron microscopy studies indicated that IgM+AnnV+PI+ apoptotic cells exhibited morphologically advanced apoptosis with marked plasma membrane disruption compared to IgM-AnnV+PI+ apoptotic cells, suggesting that access to intracellular epitopes is require"],"journal":["PloS one"],"pubmed_title":["Circulating IgM Requires Plasma Membrane Disruption to Bind Apoptotic and Non-Apoptotic Nucleated Cells and Erythrocytes."],"pmcid":["PMC4488261"],"funding_grant_id":["ST4/2011"],"pubmed_authors":["Kluth DC","Hesketh EE","Hughes J","Dransfield I"],"additional_accession":[]},"is_claimable":false,"name":"Circulating IgM Requires Plasma Membrane Disruption to Bind Apoptotic and Non-Apoptotic Nucleated Cells and Erythrocytes.","description":"Autoimmunity is associated with defective phagocytic clearance of apoptotic cells. IgM deficient mice exhibit an autoimmune phenotype consistent with a role for circulating IgM antibodies in apoptotic cell clearance. We have extensively characterised IgM binding to non-apoptotic and apoptotic mouse thymocytes and human Jurkat cells using flow cytometry, confocal imaging and electron microscopy. We demonstrate strong specific IgM binding to a subset of Annexin-V (AnnV)+PI (Propidium Iodide)+ apoptotic cells with disrupted cell membranes. Electron microscopy studies indicated that IgM+AnnV+PI+ apoptotic cells exhibited morphologically advanced apoptosis with marked plasma membrane disruption compared to IgM-AnnV+PI+ apoptotic cells, suggesting that access to intracellular epitopes is require","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2025-06-01T01:35:39.42Z","creation":"2019-03-26T23:33:30Z"},"accession":"S-EPMC4488261","cross_references":{"pubmed":["26121639"],"doi":["10.1371/journal.pone.0131849"]}}