{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Juneau RA"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["316-24"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4490236"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["212(2)"],"pubmed_abstract":["Acute gonorrhea is characterized by neutrophilic inflammation that is insufficient to clear Neisseria gonorrhoeae. Activated neutrophils release extracellular traps (NETs), which are composed of chromatin and decorated with antimicrobial proteins. The N. gonorrhoeae NG0969 open reading frame contains a gene (nuc) that encodes a putatively secreted thermonuclease (Nuc) that contributes to biofilm remodeling. Here, we report that Nuc degrades NETs to help N. gonorrhoeae resist killing by neutrophils. Primary human neutrophils released NETs after exposure to N. gonorrhoeae, but NET integrity declined over time with Nuc-containing bacteria. Recombinant Nuc and conditioned medium from Nuc-containing N. gonorrhoeae degraded human neutrophil DNA and NETs. NETs were found to have antimicrobial act"],"journal":["The Journal of infectious diseases"],"pubmed_title":["A thermonuclease of Neisseria gonorrhoeae enhances bacterial escape from killing by neutrophil extracellular traps."],"pmcid":["PMC4490236"],"funding_grant_id":["R21 AI109320","T32AI007046","R01 AI097312","T32GM007267","T32 GM007267","R01AI09320","R21 AI110889","R01AI097312","T32 AI007046","R01 AI108255","R01AI108255","R21AI110889"],"pubmed_authors":["Stevens JS","Apicella MA","Criss AK","Juneau RA"],"additional_accession":[]},"is_claimable":false,"name":"A thermonuclease of Neisseria gonorrhoeae enhances bacterial escape from killing by neutrophil extracellular traps.","description":"Acute gonorrhea is characterized by neutrophilic inflammation that is insufficient to clear Neisseria gonorrhoeae. Activated neutrophils release extracellular traps (NETs), which are composed of chromatin and decorated with antimicrobial proteins. The N. gonorrhoeae NG0969 open reading frame contains a gene (nuc) that encodes a putatively secreted thermonuclease (Nuc) that contributes to biofilm remodeling. Here, we report that Nuc degrades NETs to help N. gonorrhoeae resist killing by neutrophils. Primary human neutrophils released NETs after exposure to N. gonorrhoeae, but NET integrity declined over time with Nuc-containing bacteria. Recombinant Nuc and conditioned medium from Nuc-containing N. gonorrhoeae degraded human neutrophil DNA and NETs. NETs were found to have antimicrobial act","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jul","modification":"2025-04-05T09:02:09.901Z","creation":"2019-03-27T01:54:26Z"},"accession":"S-EPMC4490236","cross_references":{"pubmed":["25605868"],"doi":["10.1093/infdis/jiv031"]}}