{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Porchetta A"],"funding":["Associazione Italiana per la Ricerca sul Cancro","Natural Sciences and Engineering Research Council of Canada","European Research Council","European Commission Directorate-General for Research and Innovation"],"pagination":["4467-71"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4498449"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(7)"],"pubmed_abstract":["Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change."],"journal":["Nano letters"],"pubmed_title":["General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands."],"pmcid":["PMC4498449"],"funding_grant_id":["14420","436381-2013","336493"],"pubmed_authors":["Ricci F","Idili A","Porchetta A","Vallee-Belisle A"],"additional_accession":[]},"is_claimable":false,"name":"General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands.","description":"Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jul","modification":"2025-04-05T09:02:43.534Z","creation":"2019-03-27T01:54:57Z"},"accession":"S-EPMC4498449","cross_references":{"pubmed":["26053894"],"doi":["10.1021/acs.nanolett.5b00852"]}}