{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Park SC"],"funding":["NIDDK NIH HHS","NCI NIH HHS"],"pagination":["1506-14"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4499771"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(13)"],"pubmed_abstract":["A large number of cancer stem cells (CSCs) have been isolated and identified; however, none has been cultured in an unlimited manner in vitro without losing tumorigenicity and multipotency. In this study, we successfully clonogenically cultured a newly identified CD34+ liver CSC (LCSC) on feeder cells up to 22 passages (to date) without losing CSC property. Cloned CD34+ LCSC formed a round packed morphology and it could also be cryopreserved and recultured. Stem cell markers, CD34, CD117, and SOX2; normal liver stem cell markers, alpha fetoprotein, CK19, CK18, and OV6; putative CSC markers, CD44, CD133, EpCAM, and CD90; as well as CD31 were expressed in cloned CD34+ LCSC. SOX2 was the major factor in maintaining this LCSC before colonization, and interestingly, OCT4, SOX2, NAONG, Klf4, c-M"],"journal":["Stem cells and development"],"pubmed_title":["Clonogenically Culturing and Expanding CD34+ Liver Cancer Stem Cells in Vitro."],"pmcid":["PMC4499771"],"funding_grant_id":["DK075415","P30 CA093373"],"pubmed_authors":["Zeng C","Zhao M","Park SC","Zhang Y","Ramsamooj R","Nguyen NT","Eun JR","Tschudy-Seney B","Zhou H","Zern MA","Theise ND","Duan Y"],"additional_accession":[]},"is_claimable":false,"name":"Clonogenically Culturing and Expanding CD34+ Liver Cancer Stem Cells in Vitro.","description":"A large number of cancer stem cells (CSCs) have been isolated and identified; however, none has been cultured in an unlimited manner in vitro without losing tumorigenicity and multipotency. In this study, we successfully clonogenically cultured a newly identified CD34+ liver CSC (LCSC) on feeder cells up to 22 passages (to date) without losing CSC property. Cloned CD34+ LCSC formed a round packed morphology and it could also be cryopreserved and recultured. Stem cell markers, CD34, CD117, and SOX2; normal liver stem cell markers, alpha fetoprotein, CK19, CK18, and OV6; putative CSC markers, CD44, CD133, EpCAM, and CD90; as well as CD31 were expressed in cloned CD34+ LCSC. SOX2 was the major factor in maintaining this LCSC before colonization, and interestingly, OCT4, SOX2, NAONG, Klf4, c-M","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jul","modification":"2026-07-15T01:05:23.015Z","creation":"2019-03-27T01:55:00Z"},"accession":"S-EPMC4499771","cross_references":{"pubmed":["25867583"],"doi":["10.1089/scd.2015.0022"]}}