{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yoh SM"],"funding":["NIDDK NIH HHS","NIDA NIH HHS","NIAID NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["1293-1305"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4503237"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["161(6)"],"pubmed_abstract":["Dendritic cells (DCs) play a critical role in the immune response to viral infection through the facilitation of cell-intrinsic antiviral activity and the activation of adaptive immunity. HIV-1 infection of DCs triggers an IRF3-dependent innate immune response, which requires the activity of cyclic GAMP synthase (cGAS). We report the results of a targeted RNAi screen utilizing primary human monocyte-derived DCs (MDDCs) to identify immune regulators that directly interface with HIV-1-encoded features to initiate this innate response. Polyglutamine binding protein 1 (PQBP1) emerged as a strong candidate through this analysis. We found that PQBP1 directly binds to reverse-transcribed HIV-1 DNA and interacts with cGAS to initiate an IRF3-dependent innate response. MDDCs derived from Renpenning"],"journal":["Cell"],"pubmed_title":["PQBP1 Is a Proximal Sensor of the cGAS-Dependent Innate Response to HIV-1."],"pmcid":["PMC4503237"],"funding_grant_id":["U19 AI106754","R01 DA033773","U24 DK097153","P50 GM085764","P01 AI090935","P01 CA177322","P30 CA030199","R01 AI105184"],"pubmed_authors":["Yoh SM","des Portes V","Garcia-Sastre A","Konig R","Schneider M","Seifried J","De Jesus PD","Chanda SK","Germanaud D","Olivieri KC","Ruan C","Ruiz PA","Soonthornvacharin S","de Castro E","Akleh RE"],"additional_accession":[]},"is_claimable":false,"name":"PQBP1 Is a Proximal Sensor of the cGAS-Dependent Innate Response to HIV-1.","description":"Dendritic cells (DCs) play a critical role in the immune response to viral infection through the facilitation of cell-intrinsic antiviral activity and the activation of adaptive immunity. HIV-1 infection of DCs triggers an IRF3-dependent innate immune response, which requires the activity of cyclic GAMP synthase (cGAS). We report the results of a targeted RNAi screen utilizing primary human monocyte-derived DCs (MDDCs) to identify immune regulators that directly interface with HIV-1-encoded features to initiate this innate response. Polyglutamine binding protein 1 (PQBP1) emerged as a strong candidate through this analysis. We found that PQBP1 directly binds to reverse-transcribed HIV-1 DNA and interacts with cGAS to initiate an IRF3-dependent innate response. MDDCs derived from Renpenning","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jun","modification":"2025-04-19T07:18:30.119Z","creation":"2019-03-27T01:55:10Z"},"accession":"S-EPMC4503237","cross_references":{"pubmed":["26046437"],"doi":["10.1016/j.cell.2015.04.050"]}}