biostudies-literatureBatzenschlager MPHC Van GoghPHC Procope8656-60https://www.ebi.ac.uk/biostudies/studies/S-EPMC4507256biostudies-literatureUnknown112(28)Centromeres play a pivotal role in maintaining genome integrity by facilitating the recruitment of kinetochore and sister-chromatid cohesion proteins, both required for correct chromosome segregation. Centromeres are epigenetically specified by the presence of the histone H3 variant (CENH3). In this study, we investigate the role of the highly conserved γ-tubulin complex protein 3-interacting proteins (GIPs) in Arabidopsis centromere regulation. We show that GIPs form a complex with CENH3 in cycling cells. GIP depletion in the gip1gip2 knockdown mutant leads to a decreased CENH3 level at centromeres, despite a higher level of Mis18BP1/KNL2 present at both centromeric and ectopic sites. We thus postulate that GIPs are required to ensure CENH3 deposition and/or maintenance at centromeres. InProceedings of the National Academy of Sciences of the United States of AmericaArabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture.PMC450725631504VJ30746TBBerr ASchubert VKoini MALermontova IChaboute MEHoulne GSchmit ACFransz PAlioua AFuchs JBatzenschlager MRutten THerzog EfalseArabidopsis MZT1 homologs GIP1 and GIP2 are essential for centromere architecture.Centromeres play a pivotal role in maintaining genome integrity by facilitating the recruitment of kinetochore and sister-chromatid cohesion proteins, both required for correct chromosome segregation. Centromeres are epigenetically specified by the presence of the histone H3 variant (CENH3). In this study, we investigate the role of the highly conserved γ-tubulin complex protein 3-interacting proteins (GIPs) in Arabidopsis centromere regulation. We show that GIPs form a complex with CENH3 in cycling cells. GIP depletion in the gip1gip2 knockdown mutant leads to a decreased CENH3 level at centromeres, despite a higher level of Mis18BP1/KNL2 present at both centromeric and ectopic sites. We thus postulate that GIPs are required to ensure CENH3 deposition and/or maintenance at centromeres. In2015-01-01T00:00:00Z2015 Jul2026-04-08T16:46:27.906Z2019-03-27T01:55:24ZS-EPMC45072562612414610.1073/pnas.1506351112