{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Drosopoulos WC"],"funding":["NIGMS NIH HHS"],"pagination":["191-208"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4508891"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["210(2)"],"pubmed_abstract":["Based on its in vitro unwinding activity on G-quadruplex (G4) DNA, the Bloom syndrome-associated helicase BLM is proposed to participate in telomere replication by aiding fork progression through G-rich telomeric DNA. Single molecule analysis of replicated DNA (SMARD) was used to determine the contribution of BLM helicase to telomere replication. In BLM-deficient cells, replication forks initiating from origins within the telomere, which copy the G-rich strand by leading strand synthesis, moved slower through the telomere compared with the adjacent subtelomere. Fork progression through the telomere was further slowed in the presence of a G4 stabilizer. Using a G4-specific antibody, we found that deficiency of BLM, or another G4-unwinding helicase, the Werner syndrome-associated helicase WR"],"journal":["The Journal of cell biology"],"pubmed_title":["BLM helicase facilitates telomere replication during leading strand synthesis of telomeres."],"pmcid":["PMC4508891"],"funding_grant_id":["R01 GM045751","5R01-GM045751"],"pubmed_authors":["Drosopoulos WC","Kosiyatrakul ST","Schildkraut CL"],"additional_accession":[]},"is_claimable":false,"name":"BLM helicase facilitates telomere replication during leading strand synthesis of telomeres.","description":"Based on its in vitro unwinding activity on G-quadruplex (G4) DNA, the Bloom syndrome-associated helicase BLM is proposed to participate in telomere replication by aiding fork progression through G-rich telomeric DNA. Single molecule analysis of replicated DNA (SMARD) was used to determine the contribution of BLM helicase to telomere replication. In BLM-deficient cells, replication forks initiating from origins within the telomere, which copy the G-rich strand by leading strand synthesis, moved slower through the telomere compared with the adjacent subtelomere. Fork progression through the telomere was further slowed in the presence of a G4 stabilizer. Using a G4-specific antibody, we found that deficiency of BLM, or another G4-unwinding helicase, the Werner syndrome-associated helicase WR","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Jul","modification":"2025-04-26T08:15:06.109Z","creation":"2019-03-27T01:55:30Z"},"accession":"S-EPMC4508891","cross_references":{"pubmed":["26195664"],"doi":["10.1083/jcb.201410061"]}}