{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(9B)"],"submitter":["Pascaru M"],"pubmed_abstract":["In order to analyse whether a C-terminal polybasic sequence represents a nuclear localization signal (NLS) we obtained several truncated and mutant forms of protein of regerating liver (PRL)-3 and evaluated their subcellular localization as compared to the wild-type form. Our results invalidate the hypothesis that this is an NLS. We also analysed the influence of the C- and N-terminal residues on the phosphatase activity of PRL-3. Our results provide in vitro evidence that the C-terminal CAAX motif, besides directing the protein farnesylation, plays an additional regulatory role by inhibiting the catalytic efficiency of PRL-3. Taking into account the results we obtained, as well as reported data, we propose a hypothetical molecular mechanism for the nucleocytoplasmic localization and trans"],"journal":["Journal of cellular and molecular medicine"],"pagination":["3141-50"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4516473"],"repository":["biostudies-literature"],"pubmed_title":["Analysis of molecular determinants of PRL-3."],"pmcid":["PMC4516473"],"pubmed_authors":["Popescu I","Tanase C","Boeti P","Szedlacsek SE","Vacaru AM","Neagu E","Pascaru M"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of molecular determinants of PRL-3.","description":"In order to analyse whether a C-terminal polybasic sequence represents a nuclear localization signal (NLS) we obtained several truncated and mutant forms of protein of regerating liver (PRL)-3 and evaluated their subcellular localization as compared to the wild-type form. Our results invalidate the hypothesis that this is an NLS. We also analysed the influence of the C- and N-terminal residues on the phosphatase activity of PRL-3. Our results provide in vitro evidence that the C-terminal CAAX motif, besides directing the protein farnesylation, plays an additional regulatory role by inhibiting the catalytic efficiency of PRL-3. Taking into account the results we obtained, as well as reported data, we propose a hypothetical molecular mechanism for the nucleocytoplasmic localization and trans","dates":{"release":"2009-01-01T00:00:00Z","publication":"2009 Sep","modification":"2025-04-27T02:51:34.458Z","creation":"2019-03-26T23:36:54Z"},"accession":"S-EPMC4516473","cross_references":{"pubmed":["19040419"],"doi":["10.1111/j.1582-4934.2008.00591.x"]}}